Kozyra Malgorzata, Biernasiuk Anna, Malm Anna
Acta Pol Pharm. 2017 Jan;74(1):161-172.
In this work the phenolic acids in crude methanol extracts from the flowering herbs of Carluus acanthoides L. were identified. The samples containing free phenolic acids and those released after acid and alka- line hydrolyses were investigated by 2D TLC on cellulose. After purification by SPE, samples were also analyzed by RP-HPLC. Chlorogenic, protocatechuic, p- coumaric, caffeic, syringic, p-hydroxybenzoic, ferulic, vanillic, gentisic and gallic acids were detected in fractions of the methanolic extract obtained from the flow- ering herb of C. acanthoides. This is the first study concerning the qualitative analysis of phenolic compounds and antibacterial activity of fractions and aqueous, ethyl acetate, dichloromethane, acetone and methanol (50, 80 and 100% v/v) extracts of flowering herbs of C. acanthoides L. The antimicrobial activity of tested extracts was determined in vito against reference microorganisms of Gram-positive bacteria, Gram-negative bacteria and fungi belonging to yeasts. The results of this study support the medical usage of C. acanthoides L. due to its antimicrobial properties.
在本研究中，对刺果飞廉开花草本植物粗甲醇提取物中的酚酸进行了鉴定。通过纤维素二维薄层色谱法对含有游离酚酸以及经酸碱水解后释放出的酚酸的样品进行了研究。经固相萃取净化后，样品还采用反相高效液相色谱法进行了分析。在从刺果飞廉开花草本植物获得的甲醇提取物馏分中检测到了绿原酸、原儿茶酸、对香豆酸、咖啡酸、丁香酸、对羟基苯甲酸、阿魏酸、香草酸、龙胆酸和没食子酸。这是关于刺果飞廉开花草本植物酚类化合物定性分析以及其馏分和水、乙酸乙酯、二氯甲烷、丙酮和甲醇（体积分数分别为50%、80%和100%）提取物抗菌活性的首次研究。在体外测定了受试提取物对革兰氏阳性菌、革兰氏阴性菌以及属于酵母的真菌等参考微生物的抗菌活性。本研究结果支持了刺果飞廉因其抗菌特性而具有的药用价值。
