Azzouzi Djihane, Bioud Kenza, Demirtas Ibrahim, Gul Fatih, Sarri Djamel, Benayache Samir, Benayache Fadila, Mekkiou Ratiba
Unite de Recherche Valorisation des Ressources Naturelles, Molecules Bioactives et Analyses Physicochimiques et Biologiques (VARENBIOMOL), Departement de Chimie, Faculte des Sciences Exactes, Universite Constantine 1, 25000 Constantine, Algerie.. Algeria.
Comb Chem High Throughput Screen. 2016;19(10):841-846. doi: 10.2174/1574888X11666161102092319.
This study aimed to quantify phenolic compounds in ethyl acetate and n-butanol extract of Centaurea choulettiana Pomel (Asteraceae) leaves and flowers; compare the antioxidant activity of their extracts, identification and quantification of their phenolic acids.
Both organs extracts of Centaurea choulettiana Pomel were investigated and evaluated for their potential antioxidant properties using total phenolics and flavonoids content, DPPH radical scavenging and lipid peroxidation inhibition assays. HPLC-TOF/MS analyses were carried out to identify and quantify some phenolic acids.
The amounts of phenolic and flavonoid content were higher in ethyl acetate extract of leaves (325.81 ± 0.038 mgGAE and 263.73 ± 0.004 mgQE /g of extract) respectively. Besides, this extract exhibited the most powerful effect on the DPPH radical scavenging activity with (96.54%), on lipid peroxydation inhibition (64.17%). Ethyl acetate extract of leaves and flowers were found to contain almost the same phenolic compounds, with the leaves having the highest values. Chlorogenic acid was detected in the n-butanol extract of flowers with the highest concentration 17.78 mg/kg plant.
The ethyl acetate extract of leaves of Centaurea choulettiana possesses strong antioxidative properties in vitro. They are confirmed by high polyphenols and flavonoids content. The HPLC-TOF/MS analysis reveals the presence of 4-hydroxybenzoic acid, gentisic acid, chlorogenic acid, caffeic acid, vanillic acid, p-Coumaric acid, ferulic acid, salicylic acid and protocatechuic acid.
本研究旨在对菊科植物乔氏矢车菊（Centaurea choulettiana Pomel）叶和花的乙酸乙酯提取物及正丁醇提取物中的酚类化合物进行定量分析；比较其提取物的抗氧化活性，鉴定并定量其酚酸成分。
对乔氏矢车菊的叶和花提取物进行研究，并通过总酚和黄酮含量、DPPH自由基清除及脂质过氧化抑制试验评估其潜在抗氧化性能。采用高效液相色谱-飞行时间质谱（HPLC-TOF/MS）分析来鉴定和定量一些酚酸。
叶的乙酸乙酯提取物中酚类和黄酮含量分别更高（325.81±0.038mg没食子酸当量和263.73±0.004mg槲皮素当量/克提取物）。此外，该提取物对DPPH自由基清除活性最强（96.54%），对脂质过氧化抑制作用也最强（64.17%）。叶和花的乙酸乙酯提取物所含酚类化合物几乎相同，叶中的含量最高。在花的正丁醇提取物中检测到绿原酸，其最高浓度为17.78mg/kg植物。
乔氏矢车菊叶的乙酸乙酯提取物在体外具有较强的抗氧化性能。高含量的多酚和黄酮类化合物证实了这一点。HPLC-TOF/MS分析显示存在4-羟基苯甲酸、龙胆酸、绿原酸、咖啡酸、香草酸、对香豆酸、阿魏酸、水杨酸和原儿茶酸。
