Division of Microbiology and Molecular Medicine, Faculty of Medicine and Health Sciences, Linköping University, SE-58185 Linköping, Sweden.
Biotecnología Médica y Farmacéutica, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, A.C. Av. Normalistas No. 800, Col. Colinas de la Normal, 44270 Guadalajara, Jalisco, Mexico.
Vaccine. 2018 Mar 27;36(14):1811-1815. doi: 10.1016/j.vaccine.2018.02.044. Epub 2018 Feb 21.
Tuberculosis (TB) still remains as an unmet global threat. The current vaccine is not fully effective and novel alternatives are needed. Here, two vaccine candidate strains derived from BCG carrying deletions in the BCG1416c or BCG1419c genes were analysed for their capacity to modulate the cytokine/chemokine profile and granuloma formation in a human lung tissue model (LTM). We show that the clustering of monocytes, reminiscent of early granuloma formation, in LTMs infected with BCG strains was similar for all of them. However, BCGΔBCG1419c, like M. tuberculosis, was capable of inducing the production of IL-6 in contrast to the other BCG strains. This work suggests that LTM could be a useful ex vivo assay to evaluate the potential immunogenicity of novel TB vaccine candidates.
结核病（TB）仍然是一个未满足的全球威胁。目前的疫苗并非完全有效，因此需要新型替代品。在这里，我们分析了两种源自卡介苗的候选疫苗菌株，它们在含有 BCG1416c 或 BCG1419c 基因缺失的情况下，在人肺组织模型（LTM）中调节细胞因子/趋化因子谱和肉芽肿形成的能力。我们表明，在 LTM 中感染卡介苗菌株后，类似于早期肉芽肿形成的单核细胞聚集对于所有菌株都是相似的。然而，与其他卡介苗菌株不同，BCGΔBCG1419c 能够诱导 IL-6 的产生，就像结核分枝杆菌一样。这项工作表明，LTM 可以成为一种有用的体外检测方法，用于评估新型结核病疫苗候选物的潜在免疫原性。
