School of Civil Engineering and Architecture, Wuhan University of Technology, Wuhan, Hubei, 430070, China.
School of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of Technology, Wuhan, Hubei, 430070, China.
Environ Sci Pollut Res Int. 2018 May;25(13):12830-12840. doi: 10.1007/s11356-018-1527-z. Epub 2018 Feb 23.
Over the last three decades, the presence of arbuscular mycorrhizal fungi (AMF) in wetland habitats had been proven, and their roles played in wetland ecosystems and potential functions in wastewater bioremediation technical installations are interesting issues. To increase knowledge on the functions of AMF in the plant-based bioremediation of wastewater, we constructed two vertical-flow wetlands planting with Phragmites australis and investigated AMF distribution in plant roots and their roles played in purification of wastewater polluted by heavy metals (HMs), utilizing the Illumina sequencing technique. A total of 17 operational taxonomic units (OTUs) from 33,031 AMF sequences were obtained, with Glomus being the most dominant. P. australis living in the two vertical-flow constructed wetlands (CWs) harbored diverse AMF comparable with the AM fungal communities in upland habitats. The AMF composition profiles of CW1 (vegetated with non-inoculated plants) and CW2 (vegetated with mycorrhizal plants inoculated with Rhizophagus intraradices) were significantly different. CW1 (15 OTUs) harbored more diverse AMF than CW2 (7 OTUs); however, CW2 harbored much more OTU13 than CW1. In addition, a zipf species abundance distribution (SAD), which might due to the heavy overdominance of OTU13, was observed across AM fugal taxa in P. australis roots of the two CWs. CW1 and CW2 showed high (> 70%) removal capacity of HMs. CW2 exhibited significant higher Cd and Zn removal efficiencies than CW1 (CK) (p = 0.005 and p = 0.008, respectively). It was considered that AMF might play a role in HM removal in CWs.
在过去的三十年中，已经证明了丛枝菌根真菌（AMF）存在于湿地生境中，它们在湿地生态系统中的作用以及在废水生物修复技术装置中的潜在功能是有趣的问题。为了增加对 AMF 在植物修复废水中的功能的了解，我们构建了两个垂直流湿地，种植了芦苇，并利用 Illumina 测序技术调查了 AMF 在植物根系中的分布及其在重金属（HM）污染废水净化中的作用。从 33031 个 AMF 序列中获得了 17 个操作分类单元（OTU），其中 Glomus 最为优势。生活在两个垂直流人工湿地（CW）中的芦苇含有与旱地栖息地中 AM 真菌群落可比的多样 AMF。CW1（种植未接种植物）和 CW2（种植接种了 Rhizophagus intraradices 的菌根植物）的 AMF 组成谱显着不同。CW1（15 个 OTU）比 CW2（7 个 OTU）含有更多的 AMF 多样性；然而，CW2 含有比 CW1 多得多的 OTU13。此外，在两个 CW 中芦苇根系的 AM 真菌类群中观察到了一个齐普夫物种丰度分布（SAD），这可能是由于 OTU13 的严重过度优势所致。CW1 和 CW2 对 HMs 的去除率均高于 70%。CW2 对 Cd 和 Zn 的去除效率明显高于 CW1（CK）（p=0.005 和 p=0.008）。考虑到 AMF 可能在 CW 中对 HM 的去除起作用。
