浮游植物研究中的光谱和成像流式细胞术。

Spectral and Imaging Flow Cytometry in Phytoplankton Research.

作者信息

Dashkova Veronika, Clapper Jeff, Vorobjev Ivan A, Barteneva Natasha S

机构信息

National Laboratory Astana, Department of Biology, School of Science and Technology, Nazarbayev University, Astana, Kazakhstan.

Sony Biotechnology Inc., San Jose, CA, USA.

出版信息

Methods Mol Biol. 2018;1745:83-95. doi: 10.1007/978-1-4939-7680-5_5.

DOI:10.1007/978-1-4939-7680-5_5

PMID:29476464

Abstract

Spectral and imaging flow cytometry are emerging technologies that allow quantifying spectral, fluorescent, and/or morphological parameters of heterogeneous cellular populations. The protocol describes a detailed step-by-step analysis of microalgae using these techniques and examples from our laboratory (Aphanizomenon sp., Cryptomonas pyrenoidifera, and Chlorella sp.). Moreover, the chapter will be helpful to scientists who want to perform spectral flow cytometry and apply principal component analysis.

摘要

光谱流式细胞术和成像流式细胞术是新兴技术，可用于量化异质细胞群体的光谱、荧光和/或形态学参数。本方案详细介绍了使用这些技术对微藻进行逐步分析的步骤，并列举了我们实验室（水华束丝藻属、隐球藻和小球藻属）的实例。此外，本章将对希望进行光谱流式细胞术并应用主成分分析的科学家有所帮助。