Kikuta Junichi, Ishii Masaru
Department of Immunology and Cell Biology, Graduate School of Medicine, Osaka University, Osaka, Japan.
Methods Mol Biol. 2018;1763:1-9. doi: 10.1007/978-1-4939-7762-8_1.
Bone is continually remodeled by bone-resorbing osteoclasts and bone-forming osteoblasts. Although it has long been believed that bone homeostasis is tightly regulated by communication between osteoclasts and osteoblasts, the fundamental process and dynamics have remained elusive. To resolve this, we established an intravital bone imaging system using multiphoton microscopy to visualize mature osteoclasts and osteoblasts in living bone.We herein describe the methodology for visualizing the in vivo behavior of bone-resorbing osteoclasts and bone-forming osteoblasts in living bone tissues using intravital multiphoton microscopy. This approach facilitates investigation of cellular dynamics in the pathogenesis of bone-destructive disorders, such as osteoporosis and rheumatoid arthritis in vivo, and would thus be useful for evaluating the efficacy of novel anti-bone-resorptive drugs.
骨骼不断地由骨吸收破骨细胞和成骨细胞进行重塑。长期以来，人们一直认为骨稳态是由破骨细胞和成骨细胞之间的通讯严格调控的，但基本过程和动态变化仍然难以捉摸。为了解决这个问题，我们建立了一个活体骨成像系统，使用多光子显微镜来观察活骨中的成熟破骨细胞和成骨细胞。我们在此描述了使用活体多光子显微镜观察活骨组织中骨吸收破骨细胞和成骨细胞体内行为的方法。这种方法有助于研究体内骨破坏性疾病（如骨质疏松症和类风湿性关节炎）发病机制中的细胞动态变化，因此对于评估新型抗骨吸收药物的疗效将是有用的。
