Department of Nephrology, Zibo Central Hospital, Zibo, PR China.
Bioengineered. 2022 Jan;13(1):593-602. doi: 10.1080/21655979.2021.2012405.
The mechanism of renal injury after cardiopulmonary bypass is not clear, and the protective effect of microRNA-146 through mediating NF KB signaling pathway needs to be verified. The study intends to establish a rat model of cardiopulmonary bypass (CPB). MiR-146 is silenced or overexpressed by lentivirus transfection. It is divided into miR-146 inhibitors group (inhibitors), miR-146 mimics group (mimics) and sham group. It is found that the contents of Cr, bun and MDA in blood = , serum IL-1, IL-6 and TNF in mimics group are higher than those in the other two groups- α Content, apoptosis rate, ICAM-1, TNF- α, NF- κ B mRNA and NF- κ B protein decreased significantly (P < 0.05), while the content of SOD in kidney increased significantly (P < 0.05). In the inhibitors group, the above indicators showed the opposite results. Double luciferase assay showed that NF-kB was the target gene of miR-146. It can be seen that the expression of miR-146 inhibits inflammatory factors, apoptosis, oxidative stress and NF- κ the activation of B pathway promotes the repair of renal injury in CPB rats.
心肺转流后肾损伤的机制尚不清楚，需要验证 microRNA-146 通过介导 NF KB 信号通路的保护作用。本研究旨在建立心肺转流（CPB）大鼠模型。通过慢病毒转染沉默或过表达 miR-146。将其分为 miR-146 抑制剂组（inhibitors）、miR-146 模拟物组（mimics）和假手术组（sham）。结果发现，mimics 组血液中 Cr、BUN 和 MDA 的含量、血清中 IL-1、IL-6 和 TNF-α的含量均高于其他两组，细胞凋亡率、ICAM-1、TNF-α、NF-κB mRNA 和 NF-κB 蛋白的含量明显降低（P<0.05），而肾组织中 SOD 的含量明显升高（P<0.05）。在抑制剂组中，上述指标则呈现相反的结果。双荧光素酶实验表明 NF-kB 是 miR-146 的靶基因。由此可见，miR-146 的表达抑制了炎症因子、细胞凋亡、氧化应激和 NF-κB 通路的激活，促进了 CPB 大鼠肾损伤的修复。
