Sakata Asuka, Nishimura Satoshi
Research Division of Cell and Molecular Medicine, Center for Molecular Medicine, Jichi Medical University, Tochigi, Japan.
Methods Mol Biol. 2018;1763:23-28. doi: 10.1007/978-1-4939-7762-8_3.
Bone imaging using multiphoton microscopy enables dynamic observation of platelet formation in living animals. Two-photon excitation microscopy is superior to confocal microscopy as it enables deeper tissue penetration, efficient light detection, and reduced phototoxicity. Using this microscopy approach, thrombopoiesis by megakaryocytes is clearly visualized in the skull at significant depth from the surface. Here we describe our microscopy setup and dye recipe for visualization of bone marrow in the mouse skull.
使用多光子显微镜进行骨成像能够动态观察活体动物中的血小板形成过程。双光子激发显微镜优于共聚焦显微镜，因为它能够实现更深的组织穿透、高效的光检测以及降低光毒性。利用这种显微镜方法，可以在距颅骨表面一定深度处清晰地观察到巨核细胞产生血小板的过程。在此，我们描述了用于观察小鼠颅骨骨髓的显微镜设置和染料配方。
