Azkargorta Mikel, Escobes Iraide, Iloro Ibon, Elortza Felix
Proteomics Platform, ProteoRed-ISCIII, CIBERehd, CIC bioGUNE, Science and Technology Park of Bizkaia, Derio, Spain.
Methods Mol Biol. 2018;1719:59-70. doi: 10.1007/978-1-4939-7537-2_4.
Peptidomics is an emerging field focused in the analysis of endogenous peptides. Naturally occurring peptides are often endogenously produced protein fragments. Cleavage of precursor proteins by proteases generates peptides that may gain specialized functions not ascribed to their precursors, and which could reflect the state of a cell under certain physiological conditions or pathological processes.Since peptides are found in complex matrices (e.g., serum, tear, urine, cerebrospinal fluid), they need to be isolated from the matrix and concentrated before they can be analyzed on mass spectrometry. This chapter describes methods for sample preparation prior to mass spectrometry analysis. In addition, different peptide fragmentation techniques are described which are complementary when analyzing naturally occurring peptides by liquid chromatography coupled online to tandem mass spectrometry.
肽组学是一个新兴领域，专注于内源性肽的分析。天然存在的肽通常是内源性产生的蛋白质片段。蛋白酶对前体蛋白的切割产生的肽可能具有其前体所没有的特殊功能，并且可以反映细胞在某些生理条件或病理过程下的状态。由于肽存在于复杂的基质中（如血清、眼泪、尿液、脑脊液），在通过质谱分析之前，需要从基质中分离并浓缩。本章介绍了质谱分析前的样品制备方法。此外，还描述了不同的肽片段化技术，这些技术在通过液相色谱在线联用串联质谱分析天然存在的肽时具有互补性。
