Van Camp Kristien A, Baggerman Geert, Blust Ronny, Husson Steven J
Department of Biology, Systemic Physiological & Ecotoxicological Research, University of Antwerp, Antwerp, Belgium.
Flemish Institute for Technological Research (VITO), Mol, Belgium.
Methods Mol Biol. 2018;1719:241-246. doi: 10.1007/978-1-4939-7537-2_16.
A wide variety of bioactive peptides are present in all metazoan species where they govern diverse functions as small messenger molecules. In the last 15 years, mass spectrometry-based methods have identified endogenous peptides in diverse species. Mass spectrometry enables the precise peptide sequences to be determined, including the potential existence of truncated versions or the presence of post-translational modifications. Because small modifications can have a large effect on biological activity, knowledge of the actual peptide sequences paves the way for further functional studies such as analysis of neuropeptidergic signaling cascades. Zebrafish (Danio rerio) is an important animal model that is commonly used in a wide range of studies. Here we provide a detailed description of the peptide extraction procedure and peptidomics workflow for zebrafish.
在所有后生动物物种中都存在各种各样的生物活性肽，它们作为小信使分子发挥着多种功能。在过去15年里，基于质谱的方法已在不同物种中鉴定出内源性肽。质谱能够确定精确的肽序列，包括截短版本的潜在存在或翻译后修饰的存在。由于微小的修饰可能对生物活性产生很大影响，了解实际的肽序列为进一步的功能研究（如神经肽信号级联分析）铺平了道路。斑马鱼（Danio rerio）是一种重要的动物模型，广泛应用于各类研究中。在此，我们详细描述了斑马鱼的肽提取程序和肽组学工作流程。
