Ghosh S N, Shah P N, Banerji A P
Acta Cytol. 1979 Mar-Apr;23(2):169-72.
Activation of second X-chromosome by the action of estradiol-17 beta was studied with HeLa cells. The incidence of X-chromatin in estrogen-treated cells decreased remarkably from 55% to 6%. Further, heavy incorporation of 3H-uridine occurred in the X-chromatin bodies of the treated cells while no such labelling was observed in controls. There was also a manifold increase in the activity of the enzyme G-6-PD in steroid-treated cells, and this increase in enzyme activity could be inhibited by addition of actinomycin D. A nonestrogenic steroid such as testosterone had no effect on these parameters. These observations do indicate activation of second X-chromosome of HeLa cells by estradiol-17 beta.
用HeLa细胞研究了17β-雌二醇作用下第二条X染色体的激活情况。雌激素处理的细胞中X染色质的发生率从55%显著下降至6%。此外，处理后的细胞X染色质体中出现了大量3H-尿苷掺入，而对照组未观察到这种标记。类固醇处理的细胞中G-6-PD酶的活性也有多种增加，添加放线菌素D可抑制酶活性的这种增加。非雌激素类类固醇如睾酮对这些参数没有影响。这些观察结果确实表明17β-雌二醇激活了HeLa细胞的第二条X染色体。
