Delgado-Galván Cindy Jazmín, Padilla-Vaca Felipe, Montiel Fátima Berenice Ramírez, Rangel-Serrano Ángeles, Paramo-Pérez Itzel, Anaya-Velázquez Fernando, Franco Bernardo
Departamento de Biología, División de Ciencias Naturales y Exactas, Universidad de Guanajuato, Noria Alta s/n, Guanajuato, Gto. 36050, Mexico.
Departamento de Biología, División de Ciencias Naturales y Exactas, Universidad de Guanajuato, Noria Alta s/n, Guanajuato, Gto. 36050, Mexico.
Exp Parasitol. 2018 Apr;187:86-92. doi: 10.1016/j.exppara.2018.01.018. Epub 2018 Feb 21.
Entamoeba histolytica genetic organization and genome structure is complex and under intense research. The genome is fully sequenced, and several tools have been developed for the molecular study of this organism. Nevertheless, good protein tracking tags that are easy to measure and image, like the fluorescent proteins are lacking. In this report, we codon-optimized the red fluorescent protein from the coral Discosoma striata (DsRFP) for its use in E. histolytica and demonstrated functionality in vivo. We envision that this protein can be widely used for the development of transcriptional reporter systems and protein-tagging applications.
溶组织内阿米巴的遗传组织和基因组结构复杂，目前正处于深入研究阶段。该基因组已完成全序列测序，并且已经开发了多种工具用于对这种生物体进行分子研究。然而，像荧光蛋白这类易于测量和成像的优质蛋白质追踪标签却十分缺乏。在本报告中，我们对来自条纹盘珊瑚（DsRFP）的红色荧光蛋白进行了密码子优化，以便在溶组织内阿米巴中使用，并在体内证明了其功能。我们设想这种蛋白质可广泛用于转录报告系统和蛋白质标记应用的开发。
