D'Atri Valentina, Fekete Szabolcs, Stoll Dwight, Lauber Matthew, Beck Alain, Guillarme Davy
School of Pharmaceutical Sciences, University of Geneva, University of Lausanne, CMU - Rue Michel Servet 1, 1211 Geneva 4, Switzerland.
School of Pharmaceutical Sciences, University of Geneva, University of Lausanne, CMU - Rue Michel Servet 1, 1211 Geneva 4, Switzerland.
J Chromatogr B Analyt Technol Biomed Life Sci. 2018 Mar 30;1080:37-41. doi: 10.1016/j.jchromb.2018.02.026. Epub 2018 Feb 20.
Brentuximab vedotin (Adcetris) is a cysteine-linked antibody-drug conjugate (ADC) used in the treatment of Hodgkin lymphoma (HL) and systemic anaplastic large cell lymphoma (ALCL). In this study, the drug payload and glycan modifications of this ADC were simultaneously characterized using a unique LC-MS middle-up analysis, involving hydrophilic interaction chromatography (HILIC). This work demonstrates that HILIC is an effective and complementary analytical technique to reversed phase liquid chromatography (RPLC) for subunit-level characterization of immuno-conjugates.
本妥昔单抗（Adcetris）是一种半胱氨酸连接的抗体药物偶联物（ADC），用于治疗霍奇金淋巴瘤（HL）和系统性间变性大细胞淋巴瘤（ALCL）。在本研究中，使用一种独特的液相色谱-质谱（LC-MS）中间向上分析方法，包括亲水相互作用色谱（HILIC），同时对该ADC的药物有效载荷和聚糖修饰进行了表征。这项工作表明，对于免疫偶联物的亚基水平表征，HILIC是一种对反相液相色谱（RPLC）有效且互补的分析技术。
