Peng Yan Qun, Fan Ling Ling, Mao Fu Ying, Zhao Yun Sheng, Xu Rui, Yin Yu Jie, Chen Xin, Wan De Guang, Zhang Xin Hui
Ningxia Medical University Pharmacy College, 750004 Yinchuan, Ningxia, China; Ningxia Research Center of Modern Hui Medicine Engineering and Technology, 750004 Yinchuan, Ningxia, China.
Ningxia Medical University Pharmacy College, 750004 Yinchuan, Ningxia, China; Ningxia Research Center of Modern Hui Medicine Engineering and Technology, 750004 Yinchuan, Ningxia, China; Ministry of Education Key Laboratory of Modern Hui Chinese Medicine, 750004 Yinchuan, Ningxia, China.
C R Biol. 2018 Mar;341(3):152-159. doi: 10.1016/j.crvi.2018.01.007. Epub 2018 Feb 22.
Polygala tenuifolia Willd. is an important protected species used in traditional Chinese medicine. In the present study, amplified fragment length polymorphism (AFLP) markers were employed to characterize the genetic diversity in wild and cultivated P. tenuifolia populations. Twelve primer combinations of AFLP produced 310 unambiguous and repetitious bands. Among these bands, 261 (84.2%) were polymorphic. The genetic diversity was high at the species level: percentage of polymorphic loci (PPL)=84.2%, Nei's gene diversity (h)=0.3296 and Shannon's information index (I)=0.4822. Between the two populations, the genetic differentiation of 0.1250 was low and the gene flow was relatively high, at 3.4989. The wild population (PPL=81.9%, h=0.3154, I=0.4635) showed a higher genetic diversity level than the cultivated population (PPL=63.9%, h=0.2507, I=0.3688). The results suggest that the major factors threatening the persistence of P. tenuifolia resources are ecological and human factors rather than genetic. These results will assist with the design of conservation and management programs, such as in natural habitat conservation, setting the excavation time interval for resource regeneration and the substitution of cultivated for wild plants.
远志是一种重要的用于传统中药的保护物种。在本研究中，采用扩增片段长度多态性（AFLP）标记来表征野生和栽培远志种群的遗传多样性。12对AFLP引物组合产生了310条清晰且可重复的条带。在这些条带中，261条（84.2%）具有多态性。物种水平的遗传多样性较高：多态位点百分比（PPL）=84.2%，Nei基因多样性（h）=0.3296，香农信息指数（I）=0.4822。两个种群之间的遗传分化较低，为0.1250，基因流相对较高，为3.4989。野生种群（PPL=81.9%，h=0.3154，I=0.4635）的遗传多样性水平高于栽培种群（PPL=63.9%，h=0.2507，I=0.3688）。结果表明，威胁远志资源持续存在的主要因素是生态和人为因素而非遗传因素。这些结果将有助于设计保护和管理计划，例如自然栖息地保护、设定资源再生的挖掘时间间隔以及用栽培植物替代野生植物。
