University of Belgrade, Faculty of Biology, Institute of Botany and Botanical Garden "Jevremovac," 11000 Belgrade, Serbia.
Department of Botany, Faculty of Science, University of Al-Gabel Al-Garbe, Zintan, Libya.
J Dairy Sci. 2018 May;101(5):3859-3865. doi: 10.3168/jds.2017-13714. Epub 2018 Feb 22.
The essential oil of Thymus algeriensis was analyzed as a potential preservative in soft cheese. We developed a novel method to test the preserving properties of essential oil in soft cheese. Contamination incidence of Penicillium aurantiogriseum was absent after 30 d of storage at 4°C with 25 µL of essential oil added. The antimicrobial activity was tested against 8 bacteria and 8 fungi. Thymus algeriensis oil showed inhibitory activity against tested bacteria at 0.03 to 0.09 mg/mL, and bactericidal activity was achieved at 0.05 to 0.15 mg/mL. For antifungal activity, minimum inhibitory concentrations ranged between 0.01 and 0.04 mg/mL and minimum fungicidal concentrations between 0.01 and 0.04 mg/mL. Furthermore, the oil was also screened for antiradical activity using the 1,1-diphenyl-2-picrylhydrazyl assay. The results showed that the oil was active and achieved half-maximal inhibitory activity at 0.132 mg/mL. We used gas chromatography, gas chromatography-mass spectrometry, and nuclear magnetic resonance spectrometry to investigate the volatile compounds from the oil. Carvacrol was identified as the main compound in the oil, represented by 80.9% of the total constituents, followed by p-cymene (7.7%).
阿尔及利亚百里香精油作为软干酪潜在防腐剂的分析。我们开发了一种新方法来测试精油在软干酪中的防腐性能。在 4°C 下储存 30 天后，添加 25 µL 的精油可使橘青霉的污染发生率为零。抗菌活性测试针对 8 种细菌和 8 种真菌。百里香精油在 0.03 至 0.09 mg/mL 时对测试细菌表现出抑制活性，在 0.05 至 0.15 mg/mL 时具有杀菌活性。对于抗真菌活性，最小抑菌浓度范围在 0.01 至 0.04 mg/mL 之间，最小杀菌浓度范围在 0.01 至 0.04 mg/mL 之间。此外，还使用 1,1-二苯基-2-苦基肼法筛选了油的抗自由基活性。结果表明，该油具有活性，在 0.132 mg/mL 时达到半最大抑制活性。我们使用气相色谱、气相色谱-质谱和核磁共振谱法研究了油中的挥发性化合物。香芹酚被鉴定为该油中的主要化合物，占总成分的 80.9%，其次是对伞花烃（7.7%）。
