Key Laboratory of Functional Small Organic Molecule, Ministry of Education, Jiangxi Normal University, Nanchang 330022, PR China; Jiangxi Academy of Agricultural Sciences, Nanchang 330200, PR China.
Key Laboratory of Functional Small Organic Molecule, Ministry of Education, Jiangxi Normal University, Nanchang 330022, PR China; State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, PR China.
Food Chem. 2018 Jun 30;252:99-107. doi: 10.1016/j.foodchem.2018.01.086. Epub 2018 Jan 12.
Ultrasound treatment could change the conformation of β-lactoglobulin (β-Lg) and improve the glycation reaction in aqueous solution under neutral condition. However, the effect of ultrasound pretreatment on glycation of β-Lg with pentose at dry-state remains ambiguous, and the relationship between glycation and allergenicity of β-Lg with ultrasound pretreatment is unclear. This study aimed to evaluate the effect of ultrasound pretreatment on glycation and allergenicity of β-Lg. Markedly decreased allergenicity of β-Lg was observed after glycation with ribose before and after ultrasound pretreatment with the minimum found at 400 W. Orbitrap LC-MS/MS showed that the glycation degree of some peptides in glycated β-Lg with and without ultrasound pretreatment were different although the content of free amino group and molecular mass were insignificantly different. Therefore, ultrasound pretreatment promoted the reduction in allergenicity by improving the glycation extent of some glycation sites although it hardly enhanced the whole glycation degree of β-Lg.
超声处理可以改变β-乳球蛋白（β-Lg）的构象，并在中性条件下改善水溶液中的糖化反应。然而，超声预处理对β-Lg 在干燥状态下与戊糖糖化的影响尚不清楚，超声预处理对β-Lg 糖化和变应原性的关系也不清楚。本研究旨在评估超声预处理对β-Lg 糖化和变应原性的影响。在用核糖对β-Lg 进行糖化前后进行超声预处理后，发现β-Lg 的变应原性明显降低，在 400 W 时最低。Orbitrap LC-MS/MS 表明，尽管游离氨基和分子量没有显著差异，但经超声预处理和未经超声预处理的糖化β-Lg 中一些肽的糖化程度不同。因此，超声预处理通过提高一些糖化部位的糖化程度来促进变应原性的降低，尽管它几乎没有增强β-Lg 的整体糖化程度。
