LeBlond Nicholas D, Fullerton Morgan D
Department of Biochemistry, Microbiology and Immunology, University of Ottawa, Ottawa, ON, Canada.
Methods Mol Biol. 2018;1732:477-493. doi: 10.1007/978-1-4939-7598-3_30.
Macrophages are a driving force in the development and progression of atherosclerosis, a chronic condition that can lead to cardiovascular disease. In this chapter we describe methods that monitor macrophage cholesterol homeostasis such as cholesterol synthesis, uptake, and efflux, all with the use of AMPK activators and potential genetic models that could help shed light on the role of this metabolic regulator in atherosclerosis and other chronic diseases.
巨噬细胞是动脉粥样硬化发展和进展的驱动力，动脉粥样硬化是一种可导致心血管疾病的慢性病症。在本章中，我们描述了监测巨噬细胞胆固醇稳态的方法，如胆固醇合成、摄取和流出，所有这些方法都使用了AMPK激活剂以及可能有助于阐明这种代谢调节因子在动脉粥样硬化和其他慢性疾病中作用的潜在基因模型。
