Miyoshi Y
Nihon Geka Gakkai Zasshi. 1986 Oct;87(10):1335-43.
The enhancement of pulmonary metastasis by local irradiation of primary tumor was investigated using Lewis lung cancer (3LL) and C57BL mice. One million 3LL cells were implanted at foot pads of mice and 7 days after the tumor-bearing legs were irradiated locally (60 Gy). Seven days after irradiation, mice were sacrificed and pulmonary metastatic nodules were counted. So it was confirmed that the pulmonary metastasis of tumor-irradiation group was enhanced significantly compared with control group and sham radiation group. The tumor-bearing legs were amputated just after, 1 day and 3 days after irradiation and pulmonary metastatic nodules were counted 7 days after irradiation. The pulmonary metastasis of mice whose legs were amputated 3 days after irradiation were enhanced significantly compared with control, though in other 2 groups pulmonary metastasis was not enhanced. So it was speculated that the enhancement of pulmonary metastasis needs remaining of irradiated tumor in bodies of mice for a certain period. It was confirmed by Winn assay that spleen cells of mice bearing irradiated tumors had an enhancing effect on tumor cells. And by Winn assay with pretreatment of cyclophosphamide, it was speculated that the enhancing effect was originated from suppressor T lymphocytes. So it was speculated that an enhancement of pulmonary metastasis by tumor irradiation was resulted from induction of suppressor T lymphocytes.
利用Lewis肺癌（3LL）和C57BL小鼠研究了对原发肿瘤进行局部照射对肺转移的增强作用。将100万个3LL细胞接种于小鼠足垫，荷瘤腿局部照射（60 Gy）7天后，于照射7天后处死小鼠并计数肺转移结节。结果证实，与对照组和假照射组相比，肿瘤照射组的肺转移显著增强。分别于照射后即刻、照射后1天和3天切除荷瘤腿，并于照射7天后计数肺转移结节。与对照组相比，照射后3天切除荷瘤腿的小鼠肺转移显著增强，而其他两组肺转移未增强。因此推测，肺转移的增强需要照射后的肿瘤在小鼠体内留存一定时间。通过Winn试验证实，荷瘤照射小鼠的脾细胞对肿瘤细胞有增强作用。通过环磷酰胺预处理的Winn试验推测，这种增强作用源于抑制性T淋巴细胞。因此推测，肿瘤照射导致的肺转移增强是由抑制性T淋巴细胞的诱导引起的。
