Havran W L, Fitch F W
Nature. 1987;325(6099):65-7. doi: 10.1038/325065a0.
L3T4, Lyt-2 and the T-cell receptor for antigen are cell-surface molecules involved in antigen specific T cell activation. We have constructed functional murine cytolytic-helper T-cell hybrid clones to study the link between expression of cell-surface molecules and specific cell function. Three of the clones express two antigen receptors and both Lyt-2 and L3T4, normally expressed on mutually exclusive subsets of mature T lymphocytes. The pattern of lymphokines produced by the hybrid cells in response to antigen was not controlled by the specific antigen receptor; both T-cell growth factor, produced only by the helper T-cell partner, and gamma-interferon, produced only by the cytolytic T-cell partner, were secreted when either antigen receptor was stimulated. However, cytolytic activity appeared to be restricted to the recognition of antigen by the T-cell receptor of the cytolytic partner. Thus cytolysis appears to be rightly linked to the antigen receptor of the cytolytic parent but lymphokine release is not tightly linked.
L3T4、Lyt-2和抗原特异性T细胞受体是参与抗原特异性T细胞激活的细胞表面分子。我们构建了功能性小鼠溶细胞辅助性T细胞杂交克隆，以研究细胞表面分子表达与特定细胞功能之间的联系。其中三个克隆表达两种抗原受体以及Lyt-2和L3T4，它们通常在成熟T淋巴细胞相互排斥的亚群上表达。杂交细胞对抗原产生的淋巴因子模式不受特异性抗原受体的控制；当任一抗原受体受到刺激时，仅由辅助性T细胞伙伴产生的T细胞生长因子和仅由溶细胞性T细胞伙伴产生的γ干扰素都会分泌。然而，溶细胞活性似乎仅限于由溶细胞性伙伴的T细胞受体识别抗原。因此，细胞溶解似乎与溶细胞性亲本的抗原受体正确相关，但淋巴因子释放并非紧密相关。
