Forensic Science Ireland, Garda HQ, Phoenix Park, Dublin 8, Ireland.
Forensic Science Ireland, Garda HQ, Phoenix Park, Dublin 8, Ireland.
Forensic Sci Int Genet. 2018 May;34:152-161. doi: 10.1016/j.fsigen.2018.02.011. Epub 2018 Feb 13.
During an investigation, the question of interest might be whether or not a person has worn a given garment. Wearer DNA studies have contributed to our knowledge and understanding of DNA transfer and persistence on items of worn clothing. However, there is currently no extensive dataset on DNA profiling outcomes from a variety of upper garments. Therefore we investigated the DNA profiling outcomes from the collars and/or cuffs of forty-four upper garments of worn clothing and determined the quantity of wearer and non-wearer DNA recovered. Interpretable DNA profiles were more likely to be obtained from collars than cuffs (84% versus 71%). The wearer was detected in all interpretable profiles and a major profile corresponding to the wearer was the most common outcome from both collar and cuff samples (48% and 50%, respectively). There was large variation in the amount of wearer DNA recovered and the average recovered was approximately 20 ng. Usually more wearer DNA was found on collars than cuffs of the same garment and, on average, more non-wearer DNA was found on cuffs than collars. No DNA was recovered from the cuffs of two garments despite these garments being worn for three and four hours, respectively. On one occasion a non-wearer contributed more DNA to a cuff sample than the wearer. We found no correlation between wearing time and the amount of wearer DNA recovered which indicates that other factors (e.g. shedder status and/or the manner of contact between the garment and skin) have a greater influence, than wearing time, on the amount of DNA transferred. However, there was a positive correlation between wearing time and the likelihood of obtaining interpretable profiles. This work has generated data to support the evaluation of DNA evidence from clothing and we provide a case example to demonstrate this.
在调查过程中，感兴趣的问题可能是一个人是否穿了特定的衣服。穿着者 DNA 研究有助于我们了解和理解 DNA 在穿着衣物上的转移和持久性。然而，目前还没有关于各种上衣 DNA 分析结果的广泛数据集。因此，我们研究了 44 件穿着衣物的衣领和/或袖口的 DNA 分析结果，并确定了从穿着衣物上回收的穿着者和非穿着者 DNA 的数量。可解释的 DNA 图谱更有可能从衣领中获得，而不是袖口（84%比 71%）。在所有可解释的图谱中都检测到了穿着者，与穿着者相对应的主要图谱是从衣领和袖口样本中最常见的结果（分别为 48%和 50%）。从穿着衣物上回收的穿着者 DNA 数量差异很大，平均回收量约为 20ng。通常，同一衣物的衣领上比袖口上能发现更多的穿着者 DNA，而平均而言，袖口上的非穿着者 DNA 比衣领上的多。尽管这两件衣服分别穿了三个小时和四个小时，但仍有两件衣服的袖口没有提取到 DNA。有一次，一个非穿着者的 DNA 比穿着者更多地出现在袖口样本中。我们没有发现穿着时间与回收的穿着者 DNA 数量之间存在相关性，这表明其他因素（例如脱落者的状态和/或衣物与皮肤之间的接触方式）对转移的 DNA 数量的影响比穿着时间更大。然而，穿着时间与获得可解释图谱的可能性之间存在正相关。这项工作生成了支持评估衣物上 DNA 证据的数据，并提供了一个案例示例来证明这一点。
