School of Marine Sciences, Ningbo University, Ningbo 315211, China; Collaborative Innovation Center for Zhejiang Marine High-efficiency and Healthy Aquaculture, Ningbo 315211, China.
School of Marine Sciences, Ningbo University, Ningbo 315211, China; Collaborative Innovation Center for Zhejiang Marine High-efficiency and Healthy Aquaculture, Ningbo 315211, China.
Comp Biochem Physiol Part D Genomics Proteomics. 2018 Jun;26:10-19. doi: 10.1016/j.cbd.2018.02.003. Epub 2018 Feb 13.
The swimming crab (Portunus trituberculatus) has a striking capacity for limb regeneration, which has drawn the interest of many researchers. In this study, isobaric tag for relative and absolute quantitation (iTRAQ) approach was utilised to investigate protein abundance changes during limb regeneration in this species. A total of 1830 proteins were identified, of which 181 were significantly differentially expressed, with 94 upregulated and 87 downregulated. Our results highlight the complexity of limb regeneration and its regulation through cooperation of various biological processes including cytoskeletal changes, extracellular matrix (ECM) remodelling and ECM-receptor interactions, protein synthesis, signal recognition and transduction, energy production and conversion, and substance transport and metabolism. Additionally, real-time PCR confirmed that mRNA levels of differentially expressed genes were correlated with protein levels. Our results provide a basis for studying the regulatory mechanisms associated with crab limb regeneration.
三疣梭子蟹（Portunus trituberculatus）具有显著的肢体再生能力，这引起了许多研究人员的兴趣。在这项研究中，我们利用同位素标记相对和绝对定量（iTRAQ）方法研究了三疣梭子蟹肢体再生过程中蛋白质丰度的变化。共鉴定到 1830 种蛋白质，其中 181 种蛋白质表达水平有显著差异，其中 94 种上调，87 种下调。我们的结果突出了肢体再生的复杂性及其通过各种生物学过程（包括细胞骨架变化、细胞外基质（ECM）重塑和 ECM-受体相互作用、蛋白质合成、信号识别和转导、能量产生和转化以及物质运输和代谢）的调控。此外，实时 PCR 验证了差异表达基因的 mRNA 水平与蛋白质水平相关。我们的结果为研究与蟹类肢体再生相关的调控机制提供了基础。
