College of Life Science and Technology, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang Province, 163319, China.
Sci Rep. 2018 Feb 26;8(1):3580. doi: 10.1038/s41598-018-22044-y.
Staphylococcus aureus can cause different types of diseases from mild skin infections to life-threatening sepsis worldwide. Owing to the emergence and transmission of multidrug-resistant strains, developing an impactful immunotherapy especially vaccine control approach against S. aureus infections is increasingly encouraged and supported. S. aureus manganese transport protein C (MntC), which is a highly-conserved cell surface protein, can elicit protective immunity against S. aureus and Staphylococcus epidermidis. In this study, we evaluated the humoral immune response and CD4 T cell-mediated immune responses in a mouse peritonitis model. The results showed that MntC-specific antibodies conferred an essential protection for mice to reduce invasion of S. aureus, which was corroborated via the opsonophagocytic killing assay and passive immunization experiment in mice, and moreover MntC-induced Th17 played a remarkable part in preventing S. aureus infection since the MntC-induced protective immunity decreased after neutralization of IL-17 by antibody in vivo and the Th17 adoptive transferred-mice could partly resist S. aureus challenge. In conclusion, we considered that the MntC-specific antibodies and MntC-specific Th17 cells play cooperative roles in the prevention of S. aureus infection.
金黄色葡萄球菌可以在全球范围内引起从轻度皮肤感染到危及生命的败血症等不同类型的疾病。由于多药耐药株的出现和传播，人们越来越鼓励和支持开发针对金黄色葡萄球菌感染的有效免疫疗法，特别是疫苗控制方法。金黄色葡萄球菌锰转运蛋白 C（MntC）是一种高度保守的细胞表面蛋白，可引发针对金黄色葡萄球菌和表皮葡萄球菌的保护性免疫。在这项研究中，我们在小鼠腹膜炎模型中评估了体液免疫和 CD4 T 细胞介导的免疫反应。结果表明，MntC 特异性抗体为小鼠提供了重要的保护，以减少金黄色葡萄球菌的侵袭，这通过调理吞噬杀伤测定和小鼠的被动免疫实验得到了证实，此外，MntC 诱导的 Th17 在预防金黄色葡萄球菌感染中发挥了重要作用，因为在体内中和 IL-17 后，MntC 诱导的保护性免疫会降低，并且 Th17 过继转移小鼠可以部分抵抗金黄色葡萄球菌的挑战。总之，我们认为 MntC 特异性抗体和 MntC 特异性 Th17 细胞在预防金黄色葡萄球菌感染中发挥协同作用。
