Department of Oncology, The Affiliated Hospital of Qingdao University, Qingdao, Shandong 266000, P.R. China.
Breast Disease Diagnosis and Treatment Center, Qingdao Center Medical Group, Qingdao, Shandong 266000, P.R. China.
Oncol Rep. 2018 Apr;39(4):1682-1692. doi: 10.3892/or.2018.6280. Epub 2018 Feb 26.
Previous studies have revealed that HURP (also known as DLGAP5 or KIAA0008) is overexpressed in many types of human cancers, such as hepatocellular carcinoma, squamous cell bladder cancer, and transitional cell carcinoma, indicating that HURP is a putative oncoprotein that promotes carcinogenesis through various molecular mechanisms. However, the role of HURP in the pathogenesis of non‑small cell lung cancer (NSCLC) has not been reported. In the present study, we investigated the prognostic value of HURP among NSCLC patients through the GEO database. The online tool of KM‑plotter was used to identify the correlation of HURP expression and the survival of NSCLC patients. We found the HURP expression at the mRNA level was correlated with the clinicopathologic characteristics and prognosis of NSCLC patients. HURP was highly expressed in aggressive NSCLC cells, and its higher expression was associated with shorter survival. Further cytological experiments revealed that the silencing of HURP caused cell cycle arrest and inhibited the proliferation of NSCLC cells. Transwell assay showed that HURP shRNA inhibited cell migration and invasion in vitro. The bioinformatic analysis suggests that HURP promotes carcinogenesis in multiple manners. Taken together, we revealed the prognostic value of HURP in NSCLC patients and HURP may be a potential therapeutic target for NSCLC.
先前的研究表明，HURP（也称为 DLGAP5 或 KIAA0008）在多种人类癌症中过表达，例如肝细胞癌、鳞状细胞膀胱癌和移行细胞癌，这表明 HURP 是一种假定的癌蛋白，通过多种分子机制促进癌症发生。然而，HURP 在非小细胞肺癌（NSCLC）发病机制中的作用尚未报道。在本研究中，我们通过 GEO 数据库研究了 HURP 在 NSCLC 患者中的预后价值。使用在线工具 KM-plotter 来确定 HURP 表达与 NSCLC 患者生存的相关性。我们发现 HURP 在 mRNA 水平的表达与 NSCLC 患者的临床病理特征和预后相关。HURP 在侵袭性 NSCLC 细胞中高表达，其高表达与生存期缩短相关。进一步的细胞学实验表明，沉默 HURP 导致细胞周期停滞并抑制 NSCLC 细胞的增殖。Transwell 测定表明 HURP shRNA 抑制 NSCLC 细胞的迁移和侵袭。生物信息学分析表明 HURP 以多种方式促进癌症发生。综上所述，我们揭示了 HURP 在 NSCLC 患者中的预后价值，HURP 可能是 NSCLC 的潜在治疗靶点。
