Montecucco C, Schiavo G
Biochem J. 1986 Jul 1;237(1):309-12. doi: 10.1042/bj2370309.
The preparation and use of a new photoactivatable phosphatidylcholine derivative [1-palmitoyl-2-(p-benzoyl)benzoyl phosphatidylcholine] is described. The reagent is shown to be effective in labelling different membrane proteins under irradiation conditions that preserve their enzymic activity. The properties of this new phospholipid analogue suggest that it can be useful in studying several aspects of membrane structure and function.
本文描述了一种新型光可激活磷脂酰胆碱衍生物[1-棕榈酰-2-(对苯甲酰基)苯甲酰磷脂酰胆碱]的制备和应用。结果表明,该试剂在能保持其酶活性的辐照条件下,对不同膜蛋白的标记有效。这种新型磷脂类似物的特性表明,它在研究膜结构和功能的多个方面可能有用。