Sabovljević Marko S, Weidinger Marieluise, Sabovljević Aneta D, Adlassnig Wolfram, Lang Ingeborg
1Institute of Botany and Botanical Garden, Faculty of Biology,University of Belgrade,Takovska 43,11000 Belgrade,Serbia.
2Core Facility Cell Imaging and Ultrastructure Research, Faculty of Life Sciences,University of Vienna,Althanstrasse 14,A-1090 Vienna,Austria.
Microsc Microanal. 2018 Feb;24(1):69-74. doi: 10.1017/S143192761800003X.
Bryophytes are usually taken as good bioindicators. However, they represent a large group of terrestrial plants and they express an enormous range of peculiarities within the plant kingdom. With the aim to search for a common pattern of zinc binding, we established axenical in vitro cultures of a dozen bryophyte species that include hornworts, thallose, and leafy liverworts, as well as acrocarp and pleurocarp mosses. The species were grown free of contaminants for many years prior to the application of different treatments, i.e. offering Zn(II) from solid and liquid media and in combination with different anions. The localization and binding of zinc was detected by confocal microscopy using the zinc-specific dye FluoZin™-3. In one of the species, Hypnum cupressiforme (which is widely used for atmospheric heavy metal deposition studies in biomonitoring), semi-quantitative analyses of zinc were performed by energy dispersive X-ray microspectrometry (EDX) in a scanning electron microscope. The results suggest no common pattern of Zn(II) binding in different bryophyte species. Instead, the binding pattern seems to be species specific. Zinc is located in certain areas or cellular compartments, as clearly shown by the EDX measurements in H. cupressiforme.
苔藓植物通常被视为良好的生物指示物。然而，它们代表了一大类陆生植物，并且在植物界展现出极为多样的特性。为了探寻锌结合的共同模式，我们建立了十几种苔藓植物的无菌体外培养体系，这些苔藓植物包括角苔、叶状体苔类和叶状苔类，以及顶蒴藓类和侧蒴藓类。在施加不同处理（即从固体和液体培养基中提供Zn(II)并与不同阴离子结合）之前，这些物种已在无污染物的环境中生长多年。使用锌特异性染料FluoZin™-3通过共聚焦显微镜检测锌的定位和结合情况。在其中一个物种——桧叶金发藓（在生物监测中广泛用于大气重金属沉积研究）中，通过扫描电子显微镜中的能量色散X射线微光谱分析（EDX）对锌进行了半定量分析。结果表明，不同苔藓植物物种中不存在锌结合的共同模式。相反，结合模式似乎具有物种特异性。如桧叶金发藓中的EDX测量结果清楚显示，锌位于某些区域或细胞区室中。
