Ugbaja R N, Olaniyan T O, Afolabi O K, Onunkwor B O, Dosumu O A, Akinhanmi T F, Tiamiyu A Y, Aliyu-Banjo N O
Department of Biochemistry, College of Biosciences, Federal University of Agriculture, Abeokuta.
Niger J Physiol Sci. 2017 Dec 30;32(2):207-212.
To investigate the effects of vitamin C and E on electrolyte profile and the activity of acetylcholinesterase (AChE) in Aluminium (Al) chloride exposed rats, thirty-six male rats were used for this study. The animals were randomly grouped into six (n=6); group I (Control) was given normal saline. Group II (Al only) was exposed to 20mg/kg body weight (BW) of Al. Groups III (Vitamin C only) and IV (Vitamin E only) were administered 200mg/kg BW of vitamin C and vitamin E respectively. Groups V (Al + Vit C) and VI (Al + Vit E) were exposed to 20mg/kg Al and were treated with 200mg/kg vitamin C and vitamin E respectively. Al exposure resulted in a significant (P<0.05) increase in plasma calcium and erythrocyte magnesium concentrations compared with control. The erythrocyte sodium concentration of group treated with Al alone was significantly (P<0.05) higher by 2.01folds than the control group. While the two vitamins were unable to correct the disruption in calcium homeostasis, they ameliorated the intracellular levels of sodium and magnesium ions. A reduction in the activity of AChE (1378.90±130.02U/L)was observed in erythrocyte of the group exposed to Al when compared to the control (1968.80±283.72U/L). Treatment with vitamins C and E further inhibited erythrocyte AChE activity by 34% and 39% respectively compared to a 30% inhibition by Al only. Positive associations were observed between erythrocyte magnesium and blood sodium, and plasma calcium and erythrocyte sodium levels. Negative associations were however observed between plasma AChE activity and erythrocyte sodium and magnesium levels. In conclusion, vitamins C and E ameliorated ionoregulatory disruptions caused by sub-acute aluminium on only erythrocyte sodium and magnesium levels but not on plasma calcium level and erythrocyte acetylcholinesterase activity.
为研究维生素C和维生素E对氯化铝暴露大鼠电解质状况及乙酰胆碱酯酶（AChE）活性的影响，本研究选用了36只雄性大鼠。将动物随机分为六组（n = 6）；第一组（对照组）给予生理盐水。第二组（仅铝组）暴露于20mg/kg体重的铝。第三组（仅维生素C组）和第四组（仅维生素E组）分别给予200mg/kg体重的维生素C和维生素E。第五组（铝+维生素C组）和第六组（铝+维生素E组）暴露于20mg/kg铝，并分别用200mg/kg维生素C和维生素E进行治疗。与对照组相比，铝暴露导致血浆钙和红细胞镁浓度显著（P<0.05）升高。仅用铝处理的组的红细胞钠浓度比对照组显著（P<0.05）高2.01倍。虽然这两种维生素无法纠正钙稳态的破坏，但它们改善了细胞内钠和镁离子水平。与对照组（1968.80±283.72U/L）相比，暴露于铝的组的红细胞中观察到AChE活性降低（1378.90±130.02U/L）。与仅铝处理导致的30%抑制相比，维生素C和E处理分别使红细胞AChE活性进一步抑制34%和39%。在红细胞镁与血钠、血浆钙与红细胞钠水平之间观察到正相关。然而，在血浆AChE活性与红细胞钠和镁水平之间观察到负相关。总之，维生素C和E仅改善了亚急性铝引起的红细胞钠和镁水平的离子调节紊乱，而对血浆钙水平和红细胞乙酰胆碱酯酶活性无改善作用。
