Shaanxi Key Laboratory of Natural Products & Chemical Biology, College of Chemistry & Pharmacy, Northwest A&F University, Xinong Road 22, Yangling, Shaanxi 712100, PR China.
State Key Laboratory of Fine Chemicals, School of Chemistry, Dalian University of Technology, No. 2 Linggong Road, Ganjingzi District, Dalian 116023, PR China.
Bioorg Med Chem. 2018 May 1;26(8):1448-1452. doi: 10.1016/j.bmc.2017.09.026. Epub 2017 Sep 23.
A new Rhodamine B-based fluorescent probe (RBO) is successfully designed and synthesized, which is a higher selective and sensitive chemosensor for Cu than other ions. Under physiological conditions (pH = 7.0), the non emission RBO displays a rapid fluorescence increase together with a color change after addition of Cu and the detection limit is down to 28nM, which can clearly illustrate the distribution of Cu with the help of laser scanning confocal microscope in plant tissues. Eventually, it confirmed that the Cu accumulates mostly in the vascular cylinder and very less in the epidermal cells of maize roots, which is important to understand how the plants take up, transport and store in the Cu.
一种新型的罗丹明 B 基荧光探针（RBO）被成功设计和合成，它是一种比其他离子对 Cu 具有更高选择性和灵敏度的化学传感器。在生理条件下（pH=7.0），非发射的 RBO 在加入 Cu 后会迅速增加荧光强度并伴随着颜色变化，检测限低至 28nM，这可以借助激光扫描共聚焦显微镜清楚地说明 Cu 在植物组织中的分布。最终，它证实 Cu 主要积累在玉米根的维管束中，而在表皮细胞中很少，这对于了解植物如何吸收、运输和储存 Cu 是很重要的。
