Division of Life Science, The Hong Kong University of Science and Technology, Hong Kong SAR, People's Republic of China.
Guangdong Engineering Research Center for Marine Algal Biotechnology, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518060, People's Republic of China.
J Exp Biol. 2018 Apr 23;221(Pt 8):jeb167478. doi: 10.1242/jeb.167478.
The larvae of many sessile marine invertebrates go through a settlement process, during which planktonic larvae attach to a substrate and metamorphose into sessile juveniles. Larval attachment and metamorphosis (herein defined as 'settlement') are complex processes mediated by many signalling pathways. Nitric oxide (NO) signalling is one of the pathways that inhibits larval settlement in marine invertebrates across different phyla. NO is synthesized by NO synthase (NOS), which is a client of the molecular chaperone heat shock protein 90 (HSP90). In the present study, we provide evidence that NO, a gaseous messenger, regulates larval settlement of By using pharmacological bioassays and western blotting, we demonstrated that NO inhibits larval settlement of and that NO signals occur mainly in the sensory organ of swimming larvae. The settlement rate of larvae decreased after heat shock treatment. Inhibition of HSP90 induced larval settlement, and attenuated the inhibition of NO donors during larval settlement. In addition, the expression level of both HSP90 and NOS declined upon settlement. These results demonstrate that HSP90 regulates the larval settlement of by interacting with the NO pathway.
许多固着海洋无脊椎动物的幼虫都要经历一个定居过程，在此过程中，浮游幼虫附着在基质上，并变态为固着的幼体。幼虫附着和变态（这里定义为“定居”）是由许多信号通路介导的复杂过程。一氧化氮（NO）信号通路是抑制不同门海洋无脊椎动物幼虫定居的信号通路之一。NO 由一氧化氮合酶（NOS）合成，NOS 是分子伴侣热休克蛋白 90（HSP90）的客户。在本研究中，我们提供了证据表明，NO 作为一种气态信使，调节着幼虫的定居。通过药理学生物测定和 Western blot 分析，我们证明了 NO 抑制了幼虫的定居，并且 NO 信号主要发生在游动幼虫的感觉器官中。经过热休克处理后，幼虫的定居率下降。HSP90 的抑制诱导了幼虫的定居，并且减弱了 NO 供体在幼虫定居过程中的抑制作用。此外，HSP90 和 NOS 的表达水平在定居后下降。这些结果表明，HSP90 通过与 NO 通路相互作用来调节幼虫的定居。
