Dangi Priya, Khatkar B S
Department of Food Technology, Guru Jambheshwar University of Science and Technology, Hisar, Haryana 125001 India.
J Food Sci Technol. 2018 Mar;55(3):1196-1200. doi: 10.1007/s13197-017-2995-8. Epub 2018 Jan 12.
Crude glutenin of four commercial wheat varieties viz. C 306, HI 977, HW 2004 and PBW 550 of diverse origin and breadmaking quality were fractionated by size-exclusion chromatography into three fractions of decreasing molecular weights. The relative quantity of peak II, containing LMW-GS specifically, varied considerably among the varieties as reflected from their discrete SEC profiles. The area % of peak II, containing protein of interest, was maximal for C 306 (22.08%) followed by PBW 550 (15.86%). The least proportion of LMW-GS were recovered from variety HW 2004 (9.68%). As the concentration of the sample extract injected to the column increased, the resolution of the peak declined in association with the slight shifting of retention time to the higher values. The best results were obtained for variety C 306 at 100 mg protein concentration with 3 M urea buffer. Consequently, the optimized conditions for purification of LMW-GS in appreciable amounts using SEC were established.
对四个商业小麦品种（即不同来源和面包制作品质的C 306、HI 977、HW 2004和PBW 550）的粗谷蛋白进行了尺寸排阻色谱分离，分为分子量递减的三个组分。从其离散的尺寸排阻色谱图可以看出，特异性包含低分子量谷蛋白（LMW-GS）的峰II的相对含量在不同品种间差异很大。包含目标蛋白的峰II的面积百分比，C 306最大（22.08%），其次是PBW 550（15.86%）。从HW 2004品种中回收的LMW-GS比例最低（9.68%）。随着注入柱中的样品提取物浓度增加，峰的分辨率下降，同时保留时间略有向更高值偏移。在100 mg蛋白质浓度下，使用3 M尿素缓冲液对C 306品种获得了最佳结果。因此，建立了使用尺寸排阻色谱法大量纯化LMW-GS的优化条件。
