铜纳米颗粒与碳纳米管对海洋微藻 Skeletonema costatum 的联合毒性。
Combined toxicities of copper nanoparticles with carbon nanotubes on marine microalgae Skeletonema costatum.
机构信息
Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education, Ocean University of China, Qingdao, 266100, China.
Key Laboratory of Marine Ecosystem and Biogeochemistry, Second Institute of Oceanography, State Oceanic Administration, Hangzhou, 310012, China.
出版信息
Environ Sci Pollut Res Int. 2018 May;25(13):13127-13133. doi: 10.1007/s11356-018-1580-7. Epub 2018 Feb 27.
To investigate the combined toxicities of copper nanoparticles (nano-Cu) with carbon nanotubes (CNTs) on marine microalgae Skeletonema costatum, algal growth inhibition tests were carried out. Toxicities of nano-Cu with CNTs and without CNTs on microalgae were determined, respectively. Chlorophyll content and photosynthetic efficiency (ΦPSII) were determined to compare negative effects of nano-Cu with CNTs and without CNTs on photosynthesis. The concentration of Cu released by nano-Cu into the medium was determined, and interactions between nano-Cu and CNTs were analyzed to study toxic mechanisms of combined toxicities of nano-Cu with CNTs. It was found that both nano-Cu and CNTs could inhibit the growth of the microalgae; however, the toxicity of CNTs on the microalgae was far lower than that of nano-Cu. The maximum growth inhibition ratio (IR) of nano-Cu on the microalgae was 86% appearing at 96 h under 1.0 mg/L nano-Cu treatment, while the maximum IR of CNTs on the microalgae was 58% at 96 h under 200 mg/L CNT treatment. CNTs could reduce the toxicity of nano-Cu on the microalgae in processes of growth and photosynthesis. Adsorption of Cu on CNTs and aggregate between Cu and CNTs in the medium were main reasons for attenuation of toxicity of nano-Cu with adding CNTs.
为了研究铜纳米粒子（nano-Cu）与碳纳米管（CNTs）联合毒性对海洋微藻 Skeletonema costatum 的影响，进行了藻类生长抑制试验。分别测定了含有和不含有 CNTs 的 nano-Cu 对微藻的毒性。测定叶绿素含量和光合效率（ΦPSII），以比较 nano-Cu 与 CNTs 和无 CNTs 对光合作用的负面影响。测定了 nano-Cu 释放到培养基中的 Cu 浓度，并分析了 nano-Cu 与 CNTs 之间的相互作用，以研究 nano-Cu 与 CNTs 联合毒性的作用机制。结果表明，nano-Cu 和 CNTs 均可抑制微藻生长；然而，CNTs 对微藻的毒性远低于 nano-Cu。在 1.0mg/L nano-Cu 处理下，96h 时 nano-Cu 对微藻的最大生长抑制率（IR）为 86%，而在 200mg/L CNT 处理下，CNTs 对微藻的最大 IR 为 58%，在 96h 时。CNTs 在生长和光合作用过程中可降低 nano-Cu 对微藻的毒性。Cu 在 CNTs 上的吸附和 Cu 与 CNTs 在介质中的聚集是添加 CNTs 降低 nano-Cu 毒性的主要原因。
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