一种在肌球蛋白亚片段1上交换碱性轻链的方法。

FEBS Lett. 1987 Jan 19;211(1):31-4. doi: 10.1016/0014-5793(87)81268-1.

Exchange of bound alkali light chains on myosin by free alkali light chains is described. It was found that the yield of hybrid obtained was dependent on the incubation time in 4.7 M NH4Cl at pH 9.5. 60% recovery of S1(A1) from S1(A2) was obtained using only a 2-fold molar excess of A1 over S1(A2).

描述了游离碱性轻链与肌球蛋白上结合的碱性轻链的交换。发现所获得的杂交体产量取决于在pH 9.5的4.7 M氯化铵中的孵育时间。仅使用比S1(A2)摩尔过量两倍的A1，就从S1(A2)中获得了60%的S1(A1)回收率。