Iasimone F, Zuccaro G, D'Oriano V, Franci G, Galdiero M, Pirozzi D, De Felice V, Pirozzi F
Bioscience and Territory Department, University of Molise, C. da Fonte Lappone, 86090 Pesche (IS), Italy E-mail:
Department of Chemical, Materials and Production Engineering, University of Naples Federico II, P. V. Tecchio, 80, 80125 Naples, Italy.
Water Sci Technol. 2018 Feb;77(3-4):1062-1071. doi: 10.2166/wst.2017.620.
A mixed culture of oleaginous yeast Lipomyces starkeyi and wastewater native microalgae (mostly Scenedesmus sp. and Chlorella sp.) was performed to enhance lipid and biomass production from urban wastewaters. A 400 L raceway pond, operating outdoors, was designed and used for biomass cultivation. Microalgae and yeast were inoculated into the cultivation pond with a 2:1 inoculum ratio. Their concentrations were monitored for 14 continuous days of batch cultivation. Microalgal growth presented a 3-day initial lag-phase, while yeast growth occurred in the first few days. Yeast activity during the microalgal lag-phase enhanced microalgal biomass productivity, corresponding to 31.4 mgTSS m d. Yeast growth was limited by low concentrations in wastewater of easily assimilated organic substrates. Organic carbon was absorbed in the first 3 days with a 3.7 mgC L d removal rate. Complete nutrient removal occurred during microalgal linear growth with 2.9 mgN L d and 0.96 mgP L d removal rates. Microalgal photosynthetic activity induced high pH and dissolved oxygen values resulted in natural bactericidal and antifungal activity. A 15% lipid/dry weight was measured at the end of the cultivation time. Fatty acid methyl ester (FAME) analysis indicated that the lipids were mainly composed of arachidic acid.
进行了产油酵母斯达氏油脂酵母与废水本地微藻（主要是栅藻属和小球藻属）的混合培养，以提高城市污水中脂质和生物质的产量。设计了一个400升的室外跑道式池塘并用于生物质培养。微藻和酵母以2:1的接种比例接种到培养池中。在连续14天的分批培养过程中监测它们的浓度。微藻生长呈现出3天的初始滞后期，而酵母生长在最初几天就开始了。微藻滞后期的酵母活性提高了微藻生物质生产力，相当于31.4 mgTSS·m⁻²·d⁻¹。酵母生长受到废水中易同化有机底物低浓度的限制。前3天有机碳的吸收速率为3.7 mgC·L⁻¹·d⁻¹。在微藻线性生长期间实现了完全营养物去除，氮去除率为2.9 mgN·L⁻¹·d⁻¹，磷去除率为0.96 mgP·L⁻¹·d⁻¹。微藻的光合活性导致高pH值和溶解氧值，产生了天然的杀菌和抗真菌活性。培养结束时测得脂质含量为15%（干重）。脂肪酸甲酯（FAME）分析表明，脂质主要由花生酸组成。
