Codo Carla Regina Bianchi, Caldas Jamil Pedro de Siqueira, Peixoto Rafaella Regina Alves, Sanches Vitor Lacerda, Guiraldelo Tamara Cristina, Cadore Solange, Marba Sérgio Tadeu Martins
Faculdade de Ciências Médicas, Universidade Estadual de Campinas, Campinas, SP, Brasil.
Instituto de Química, Universidade Estadual de Campinas, Campinas, SP, Brasil.
Rev Paul Pediatr. 2018 Apr-Jun;36(2):141-147. doi: 10.1590/1984-0462/;2018;36;2;00015. Epub 2018 Feb 19.
To determine and compare the concentrations of electrolytes and minerals in three different types of maternal milk samples: term donor milk before pasteurization, term donor milk after pasteurization and raw milk of mothers of preterm newborns at bedside.
Descriptive cross-sectional study. Concentrations of calcium (Ca), phosphorous (P), magnesium (Mg), sodium (Na) and potassium (K) were measured in random samples of three human breast milk groups. Samples were analyzed using acid mineralization assisted by microwave radiation and further analysis by inductively coupled plasma optical emission spectrometry. Concentrations were expressed in mg/L, described as mean and standard deviation. The one-way ANOVA and Tukey's post-test were applied to determine the variability between the means of each group. Significance level was set at 5%.
There was a significant reduction in the content of Ca (259.4±96.8 vs. 217.0±54.9; p=0.003), P (139.1±51.7 vs. 116.8±33.3; p=0.004) and K (580.8±177.1 vs. 470.9±109.4; p<0.0001) in donor maternal milk before and after pasteurization. Samples of raw milk presented higher contents of Na than the donated milk (twice). The elements P and Ca would only reach the daily intake levels recommended by the European Society of Pediatric Gastroenterology, Hepatology and Nutrition if at least 60 mL of milk could be offered every 3 hours. Mg levels were not different between the three groups.
There was a significant reduction in Ca, P and K levels in samples after pasteurization. The Na value in raw milk, collected at bedside, was higher than in the samples of donor's milk before pasteurization.
测定并比较三种不同类型母乳样本中的电解质和矿物质浓度，这三种样本分别为巴氏杀菌前的足月儿捐赠母乳、巴氏杀菌后的足月儿捐赠母乳以及床边早产新生儿母亲的初乳。
描述性横断面研究。对三组人母乳样本的随机样本测量钙（Ca）、磷（P）、镁（Mg）、钠（Na）和钾（K）的浓度。样本采用微波辐射辅助酸矿化法进行分析，并通过电感耦合等离子体发射光谱法进一步分析。浓度以mg/L表示，描述为均值和标准差。应用单因素方差分析和Tukey事后检验来确定每组均值之间的差异。显著性水平设定为5%。
巴氏杀菌前后捐赠母乳中Ca（259.4±96.8对217.0±54.9；p=0.003）、P（139.1±51.7对116.8±33.3；p=0.004）和K（580.8±177.1对470.9±109.4；p<0.0001）的含量显著降低。初乳样本中的Na含量比捐赠母乳高（两倍）。只有每3小时提供至少60 mL的母乳，元素P和Ca才能达到欧洲儿科胃肠病学、肝病学和营养学会推荐的每日摄入量水平。三组之间Mg水平无差异。
巴氏杀菌后样本中Ca、P和K水平显著降低。床边采集的初乳中的Na值高于巴氏杀菌前捐赠母乳样本中的Na值。
