Tami J A, Urso O E, Krolick K A
J Immunol. 1987 Feb 1;138(3):732-8.
A panel of thirty cloned rat-mouse T cell hybridomas was prepared by fusion of acetylcholine receptor (AChR)-reactive rat T cells with the mouse thymoma BW5147. The T cell hybrids were demonstrated to be AChR reactive by their ability to secrete IL 2 in response to either AChR itself or by purified AChR subunits (alpha,beta,gamma, or delta). Various patterns of AChR subunit reactivity were observed, suggesting a predominant recognition of the alpha subunit, and also a considerable cross-reactivity from one subunit to another.
通过将乙酰胆碱受体（AChR）反应性大鼠T细胞与小鼠胸腺瘤BW5147融合，制备了一组30个克隆的大鼠-小鼠T细胞杂交瘤。这些T细胞杂交瘤通过其对AChR本身或纯化的AChR亚基（α、β、γ或δ）作出反应分泌白细胞介素2的能力，被证明具有AChR反应性。观察到了AChR亚基反应性的各种模式，表明主要识别α亚基，并且从一个亚基到另一个亚基也存在相当大的交叉反应性。
