Clarke L E, Messer L I, Greenwood N M, Wisher M H
Mol Biochem Parasitol. 1987 Jan 2;22(1):79-87. doi: 10.1016/0166-6851(87)90071-5.
Eimeria are the causative agents of coccidosis, a disease which is of world wide economic importance in the poultry industry. Immunity resulting from infection is species-specific and both antibody and cell-mediated responses have been implicated. As an initial step in the development of a genetically-engineered vaccine against coccidiosis, libraries of EcoRI-digested genomic DNA from E. tenella have been constructed in Escherichia coli using the expression vector lambda amp3. Screening of the libraries with serum from chickens immunized by infection has identified at least 24 different recombinant phage which produce eimeria antigens fused to beta-galactosidase. A significant proportion of the Eimeria DNA inserts cross-hybridise with each other and contain sequences which are highly represented in the genome. The identification of these clones will enable the isolation of intact genes from E. tenella DNA and facilitate detailed analysis of the antigens and immune responses.
艾美耳球虫是球虫病的病原体，这种疾病在全球家禽业中具有重要的经济意义。感染所产生的免疫力具有种特异性，抗体和细胞介导的反应都与之相关。作为开发抗球虫病基因工程疫苗的第一步，已使用表达载体λamp3在大肠杆菌中构建了来自柔嫩艾美耳球虫的经EcoRI消化的基因组DNA文库。用感染免疫的鸡的血清筛选文库，已鉴定出至少24种不同的重组噬菌体，它们产生与β-半乳糖苷酶融合的艾美耳球虫抗原。相当一部分艾美耳球虫DNA插入片段彼此交叉杂交，并包含在基因组中高度富集的序列。这些克隆的鉴定将有助于从柔嫩艾美耳球虫DNA中分离完整基因，并促进对抗原和免疫反应的详细分析。
