茉莉酸甲酯和水杨酸提高了……愈伤组织培养物中熊果酸和齐墩果酸的产量。 （原文中“of”后面内容缺失）
Methyl Jasmonate and Salicylic Acid Enhanced the Production of Ursolic and Oleanolic Acid in Callus Cultures of .
作者信息
Vergara Martínez Víctor M, Estrada-Soto Samuel E, Arellano-García José de Jesús, Rivera-Leyva Julio C, Castillo-España Patricia, Flores Angélica Flores, Cardoso-Taketa Alexandre T, Perea-Arango Irene
机构信息
Centro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Av. Universidad 1001, Col. Chamilpa, Cuernavaca, Morelos, México.
Facultad de Farmacia, Universidad Autónoma del Estado de Morelos, Av. Universidad 1001, Col. Chamilpa, Cuernavaca, Morelos, México.
出版信息
Pharmacogn Mag. 2018 Jan;13(Suppl 4):S886-S889. doi: 10.4103/pm.pm_77_17. Epub 2018 Jan 31.
BACKGROUND
The production of triterpenes from plants for pharmacological purposes varies in concentration, due to genetic and environmental factors. culture enables the control and increase of these bioactive molecules.
OBJECTIVE
To evaluate the effect of plant growth regulators and elicitors in the induction of calli and the production of ursolic acid (UA) and oleanolic acid (OA) in .
MATERIALS AND METHODS
Leaf explants were exposed for the induction of calli at different concentrations and combinations of 2,4-dichlorophenoxyacetic acid (2,4-D) and 6-benzylaminopurine (BAP). Methyl jasmonate (MJ) and salicylic acid were used as elicitors. High-performance liquid chromatography method was used to quantify UA and OA content in each treatment.
RESULTS
Treatment with 3.0 mg/L of 2,4-D and 0.1 mg/L of BAP produced the best results for calli induction and production of UA (1.57 mg/g dry weight [DW]) and OA (1.13 mg/g DW). Both elicitors facilitated the accumulation of triterpenes.
CONCLUSION
The combination of auxins and cytokinins showed favorable results for the induction of calli. Variation concerning the accumulation of UA and OA was observed between treatments. MJ increased the production of triterpenes five times after 8 h of exposure, compared to control treatment. There is a greater accumulation of UA (16.58 mg/g DW) and OA (1.94 mg/g DW) in leaves of wild plants.
SUMMARY
Callus cultures of were obtained from leaf explants treated with 2,4-dichlorophenoxyacetic acid and 6-bencylaminopurineResulting cultures were elicited with methyl jasmonate (MJ) and salicylic acid to increase the production of the triterpenes, ursolic acid (UA), and oleanolic acid (OA)The cultures elicited with MJ increased the production of UA and OA five times, as compared to the control. 2,4-D: 2,4-dichlorophenoxyacetic acid, BAP: 6-benzylaminopurine, DW: Dry weight, MJ: Methyl jasmonate, OA: Oleanolic acid, PGRs: Plant growth regulators, UA: Ursolic acid, SA: Salicylic acid.
背景
由于遗传和环境因素，用于药理学目的的植物三萜类化合物产量在浓度上存在差异。组织培养能够控制并增加这些生物活性分子的产量。
目的
评估植物生长调节剂和诱导子对愈伤组织诱导以及罗汉果中熊果酸（UA）和齐墩果酸（OA）产量的影响。
材料和方法
将叶片外植体置于不同浓度及组合的2,4-二氯苯氧乙酸（2,4-D）和6-苄基腺嘌呤（BAP）中诱导愈伤组织。茉莉酸甲酯（MJ）和水杨酸用作诱导子。采用高效液相色谱法对各处理中UA和OA的含量进行定量分析。
结果
用3.0 mg/L的2,4-D和0.1 mg/L的BAP处理对愈伤组织诱导以及UA（1.57 mg/g干重[DW]）和OA（1.13 mg/g DW）的产量产生了最佳效果。两种诱导子均促进了三萜类化合物的积累。
结论
生长素和细胞分裂素的组合对愈伤组织诱导显示出良好效果。各处理间观察到UA和OA积累存在差异。与对照处理相比，MJ处理8小时后三萜类化合物产量增加了五倍。野生植物叶片中UA（16.58 mg/g DW）和OA（1.94 mg/g DW）积累量更高。
总结
罗汉果愈伤组织培养物是由用2,4-二氯苯氧乙酸和6-苄基腺嘌呤处理的叶片外植体制得。所得培养物用茉莉酸甲酯（MJ）和水杨酸诱导以增加三萜类化合物、熊果酸（UA）和齐墩果酸（OA）的产量。与对照相比，用MJ诱导的培养物中UA和OA产量增加了五倍。2,4-D：2,4-二氯苯氧乙酸，BAP：6-苄基腺嘌呤，DW：干重，MJ：茉莉酸甲酯，OA：齐墩果酸, PGRs：植物生长调节剂，UA：熊果酸，SA：水杨酸
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