根部的液相色谱-质谱分析及其对人癌细胞系的细胞毒性
Liquid Chromatography Mass Spectrometry Analysis and Cytotoxicity of Roots against Human Cancer Cell Lines.
作者信息
Khan Kashif Maqbool, Nahar Lutfun, Mannan Abdul, Arfan Muhammad, Khan Ghazanfar Ali, Al-Groshi Afaf, Evans Andrew, Dempster Nicola M, Ismail Fyaz M D, Sarker Satyajit D
机构信息
School of Pharmacy and Bimolecular Sciences, Medicinal Chemistry and Natural Products Research Group, Liverpool John Moores University, England, UK.
Department of Pharmacy, COMSATS Institute of Information Technology, Abbottabad, Pakistan.
出版信息
Pharmacogn Mag. 2018 Jan;13(Suppl 4):S890-S894. doi: 10.4103/pm.pm_136_17. Epub 2018 Jan 31.
BACKGROUND
Roxb. (Asparagaceae), is native to the Himalayas. This plant has been used in the prevention and effective treatment of various forms of cancers.
OBJECTIVE
This paper reports, for the first time, on the cytotoxicity of the methanol (MeOH) extract of the roots of and its solid-phase extraction (SPE) fractions against four human carcinoma cell lines and LC-ESI-QTOF-MS analysis of the SPE fractions.
MATERIALS AND METHODS
Finely powdered roots of were macerated in methanol and extracted through SPE using gradient solvent system (water: methanol) proceeded for analysis on LC-ESI-QTOF-MS and cytotoxicity against four human carcinoma cell lines: breast (MCF7), liver (HEPG2), lung (A549), and urinary bladder (EJ138), using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide assay.
RESULTS
The MeOH extract and four SPE fractions exhibited cytotoxicity against all cell lines with the IC values ranging from 6 to 79 μg/mL. As observed in other species, the presence of saponins and sapogenins in the SPE fractions was evident in the liquid chromatography-mass spectrometry data.
CONCLUSION
It is reasonable to assume that the cytotoxicity of the MeOH extract of the roots of and its SPE fractions, at least partly, due to the presence of saponins and their aglycones. This suggests that could be exploited as a potential source of cytotoxic compounds with putative anticancer potential.
SUMMARY
The MeOH extract and all solid-phase extraction (SPE) fractions exhibited various levels of cytotoxicity against all cell lines with the IC values ranging from 6 to 79 μg/mLThe presence of saponins and sapogenins in the SPE fractions was evident in the Liquid chromatography-mass spectrometry dataDue to the presence of saponins and their aglycones, suggest that could be exploited as a potential source of cytotoxic compounds with putative anticancer potential. SPE: Solid-phase extraction, MCF7: Breast cancer cell line, HEPG2: Liver cancer cell line, A549: Lung liver cancer cell line, EJ138: Urinary bladder cancer cell line, MTT: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide, LC-MS: Liquid chromatography-mass spectrometry.
背景
Roxb.（天门冬科）原产于喜马拉雅山脉。这种植物已被用于预防和有效治疗各种癌症。
目的
本文首次报道了该植物根甲醇提取物及其固相萃取（SPE）馏分对四种人类癌细胞系的细胞毒性以及对SPE馏分的液相色谱 - 电喷雾离子化 - 四极杆飞行时间质谱（LC - ESI - QTOF - MS）分析。
材料与方法
将该植物的根磨成细粉，用甲醇浸渍，并使用梯度溶剂系统（水：甲醇）通过SPE进行萃取，然后进行LC - ESI - QTOF - MS分析以及对四种人类癌细胞系（乳腺癌（MCF7）、肝癌（HEPG2）、肺癌（A549）和膀胱癌（EJ138））进行细胞毒性检测，采用3 -（4,5 - 二甲基噻唑 - 2 - 基）- 2,5 - 二苯基四氮唑溴盐检测法。
结果
甲醇提取物和四个SPE馏分对所有细胞系均表现出细胞毒性，IC值范围为6至79μg/mL。正如在其他该属物种中观察到的那样，液相色谱 - 质谱数据中SPE馏分中皂苷和皂苷元的存在很明显。
结论
有理由认为该植物根甲醇提取物及其SPE馏分的细胞毒性至少部分归因于皂苷及其苷元的存在。这表明该植物可被开发为具有潜在抗癌潜力的细胞毒性化合物的潜在来源。
总结
甲醇提取物和所有固相萃取（SPE）馏分对所有细胞系均表现出不同程度的细胞毒性，IC值范围为6至79μg/mL。液相色谱 - 质谱数据中SPE馏分中皂苷和皂苷元的存在很明显。由于皂苷及其苷元的存在，表明该植物可被开发为具有潜在抗癌潜力的细胞毒性化合物的潜在来源。SPE：固相萃取，MCF7：乳腺癌细胞系，HEPG2：肝癌细胞系，A549：肺癌细胞系，EJ138：膀胱癌细胞系，MTT：3 -（4,5 - 二甲基噻唑 - 2 - 基）- 2,5 - 二苯基四氮唑溴盐，LC - MS：液相色谱 - 质谱
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