miRNA200a调控二氧化硅诱导的小鼠肺上皮细胞中Wnt/β-连环蛋白信号通路的实验研究
[Experimental study of miRNA200a regulating Wnt/β-catenin signaling pathway in silica-induced mouse lung epithelial cells].
作者信息
Wang X, Zeng Q, Yang X Y, Liu J, Liu Y T
机构信息
Tianjin Centers for Disease Control and Prevention. Tianjin 300011, China.
出版信息
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2018 Jan 20;36(1):47-50. doi: 10.3760/cma.j.issn.1001-9391.2018.01.014.
To observe the effect of overexpression of miRNA200a (miR-200a) recombinant lentivirus on the expression of Wnt/β-catenin signaling pathway in mouse lung epithelial cell line MLE-12 induced by silica (SiO(2)) . The mice were divided into SiO(2) control group (SiO(2)) , virus control group (SiO(2)+Lv-NC) group and overexpressing miR-200a virus group (SiO(2)+Lv-miR-200a). The expression of β-catenin, MMP2, MMP9, TCF-4 and Cyclin D1 mRNA and protein were detected by realtime-PCR and western blot after incubating cells for 18 h stimulating at the final concentration of 200 μg/ml of SiO(2). The expression of miR-200a in MLE-12 cells of SiO(2)+Lv-miR-200 a group was significantly higher than that in SiO(2) group and SiO(2)+Lv-NC group. The mRNA and protein expression of β-catenin, MMP2, MMP9, TCF-4 and Cyclin D1 in MLE-12 cells of SiO(2)+Lv-miR-200a group were significantly lower than those in SiO(2) group and SiO(2)+Lv-NC group (<0.05) . Overexpression of miR-200a can inhibit the expression of related genes of Wnt/β-catenin signaling pathway in silica-induced mouse lung epithelial cells.
观察miRNA200a（miR-200a）重组慢病毒过表达对二氧化硅（SiO₂）诱导的小鼠肺上皮细胞系MLE-12中Wnt/β-连环蛋白信号通路表达的影响。将小鼠分为SiO₂对照组（SiO₂）、病毒对照组（SiO₂+Lv-NC）组和过表达miR-200a病毒组（SiO₂+Lv-miR-200a）。在终浓度为200μg/ml的SiO₂刺激细胞18小时后，通过实时荧光定量PCR和蛋白质印迹法检测β-连环蛋白、基质金属蛋白酶2（MMP2）、基质金属蛋白酶9（MMP9）、转录因子4（TCF-4）和细胞周期蛋白D1 mRNA及蛋白的表达。SiO₂+Lv-miR-200a组MLE-12细胞中miR-200a的表达明显高于SiO₂组和SiO₂+Lv-NC组。SiO₂+Lv-miR-200a组MLE-12细胞中β-连环蛋白、MMP2、MMP9、TCF-4和细胞周期蛋白D1的mRNA及蛋白表达明显低于SiO₂组和SiO₂+Lv-NC组（<0.05）。miR-200a过表达可抑制二氧化硅诱导的小鼠肺上皮细胞中Wnt/β-连环蛋白信号通路相关基因的表达。
Zotero 插件