激肽释放酶的新型合成底物及其应用。
New synthetic substrate for kallikrein and its application.
作者信息
Fujii S, Hitomi Y, Kimura K, Ishii M, Kurumi M, Aoyama T
出版信息
Adv Exp Med Biol. 1986;198 Pt A:299-308. doi: 10.1007/978-1-4684-5143-6_41.
We developed a new synthetic substrate, Pro-Phe-Arg-alpha-naphthyl ester, for kallikrein. We found that this substrate had higher specificity and sensitivity for kallikrein and was applied for the preparation of zymogram and for the histochemical demonstration. With Pro-Phe-Arg-alpha-NE as substrate, the minimum detectable concentration of human urinary kallikrein was about 0.001 KU and then kallikrein could be determined with 25 microliter of human urine. We also found the possibility that occurring of abnormalities during pregnancy were predicted by the determination of urinary kallikrein of pregnants. Zymograms were prepared for various kinds of kallikrein using this substrate. The localization of kallikrein-like enzyme in rat kidneys was defined by the application this substrate for histochemistry. Moreover, cytochemical demonstrations of leucocytes in human blood were done using Ts-Lys-alpha-NE and Ac-Tyr-alpha-NE.
我们开发了一种用于激肽释放酶的新型合成底物——脯氨酰-苯丙氨酰-精氨酰-α-萘酯。我们发现这种底物对激肽释放酶具有更高的特异性和敏感性，并将其应用于酶谱的制备和组织化学显示。以脯氨酰-苯丙氨酰-精氨酰-α-萘酯为底物，人尿激肽释放酶的最低可检测浓度约为0.001KU，然后用25微升人尿即可测定激肽释放酶。我们还发现通过测定孕妇尿激肽释放酶来预测孕期异常情况的可能性。使用这种底物为各种激肽释放酶制备了酶谱。通过将这种底物应用于组织化学确定了大鼠肾脏中类激肽释放酶的定位。此外，使用甲苯磺酰-赖氨酰-α-萘酯和乙酰-酪氨酰-α-萘酯对人血中的白细胞进行了细胞化学显示。
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