利用CLARITY和CUBIC技术对死后人类大脑进行3D成像。

3D imaging in the postmortem human brain with CLARITY and CUBIC.

作者信息

Ando Kunie, Laborde Quentin, Brion Jean-Pierre, Duyckaerts Charles

机构信息

Laboratory of Histology, Neuroanatomy and Neuropathology, ULB Neuroscience Institute, Université Libre de Bruxelles, Brussels, Belgium; Laboratoire de Neuropathologie Escourolle, Hôpital de la Pitié-Salpêtrière, Paris, France.

Laboratoire de Neuropathologie Escourolle, Hôpital de la Pitié-Salpêtrière, Paris, France.

出版信息

Handb Clin Neurol. 2018;150:303-317. doi: 10.1016/B978-0-444-63639-3.00021-9.

DOI:10.1016/B978-0-444-63639-3.00021-9

PMID:29496149

Abstract

Recent innovations in tissue clearing and imaging technologies have enabled us to analyse biological systems directly in three-dimensions using thick samples. In this review, we discuss two of these recently reported tissue-clearing technologies (CLARITY and CUBIC) that are compatible with archival formalin-fixed human brain materials that have been fixed in formalin for a long period of time. We will discuss the pros and cons of these two technologies, examples of visualisation of Alzheimer neuropathological hallmarks and the exact protocols that we regularly use in the laboratory.

摘要

组织透明化和成像技术的最新创新使我们能够使用厚样本直接在三维空间中分析生物系统。在本综述中，我们讨论了最近报道的两种与长期保存在福尔马林中的存档福尔马林固定人脑材料兼容的组织透明化技术（CLARITY和CUBIC）。我们将讨论这两种技术的优缺点、阿尔茨海默病神经病理学特征的可视化示例以及我们实验室经常使用的具体方案。