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基因在宫颈鳞状细胞癌体外及体内生物学行为中的作用。

The role of gene on the biological behavior of squamous cervical cancer in vitro and in vivo.

作者信息

Zhang Wenwen, Chen Miaomiao, Cheng Huihui, Shen Qi, Wang Ying, Zhu Xueqiong

机构信息

Department of Obstetrics and Gynecology, the Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, People's Republic of China.

出版信息

Cancer Manag Res. 2018 Feb 16;10:323-338. doi: 10.2147/CMAR.S153036. eCollection 2018.

DOI:10.2147/CMAR.S153036
PMID:29497331
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5818869/
Abstract

OBJECTIVE

The objective of the study was to explore the role of gene on the biological behavior of squamous cervical cancer.

METHODS

Differential transcription in gene between human papillomavirus (HPV)-positive and negative cervical cancer groups was identified, and the relationship between gene and () genes were explored using The Cancer Genome Atlas database. Subsequently, the role of calgranulin B on the cell proliferation, apoptosis, invasion and migration was investigated, through overexpression and/or underexpression of calgranulin B in cervical cancer cells. In addition, the effect of calgranulin B on the growth of the cervical cancer was studied via constructing xenograft model in BALB/c nude mice that either overexpressed or underexpressed calgranulin B.

RESULTS

gene transcription in cervical cancer was highly correlated with the high-risk HPV-16 and HPV-45. In addition, overexpression of calgranulin B increased cell proliferation, invasion and migration, whereas it did not significantly affect cell apoptosis. This effect was also confirmed by calgranulin B knockdown assay. Additionally, we found that the transcription of gene was negatively correlated with and genes, but positively associated with genes in cervical cancer. Furthermore, calgranulin B significantly promoted the growth of cervical cancer in vivo.

CONCLUSION

Calgranulin B promotes cell proliferation, migration and invasion of squamous cervical cancer, possibly via regulation of MMPs. Whether there are synergistic actions between calgranulin B and HPV-16/HPV-45 infection on the squamous cervical carcinogenesis or progression need further study.

摘要

目的

本研究旨在探讨基因在宫颈鳞状细胞癌生物学行为中的作用。

方法

鉴定人乳头瘤病毒（HPV）阳性和阴性宫颈癌组之间基因的差异转录，并使用癌症基因组图谱数据库探索该基因与（）基因之间的关系。随后，通过在宫颈癌细胞中过表达和/或低表达钙粒蛋白B，研究其对细胞增殖、凋亡、侵袭和迁移的作用。此外，通过在过表达或低表达钙粒蛋白B的BALB/c裸鼠中构建异种移植模型，研究钙粒蛋白B对宫颈癌生长的影响。

结果

宫颈癌中的基因转录与高危HPV-16和HPV-45高度相关。此外，钙粒蛋白B的过表达增加了细胞增殖、侵袭和迁移，而对细胞凋亡没有显著影响。钙粒蛋白B敲低试验也证实了这一作用。此外，我们发现宫颈癌中基因的转录与和基因呈负相关，但与基因呈正相关。此外，钙粒蛋白B在体内显著促进了宫颈癌的生长。

结论

钙粒蛋白B可能通过调节基质金属蛋白酶促进宫颈鳞状细胞癌的细胞增殖、迁移和侵袭。钙粒蛋白B与HPV-16/HPV-45感染在宫颈鳞状细胞癌发生或进展过程中是否存在协同作用有待进一步研究。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e5f7/5818869/5b9ef91feb00/cmar-10-323Fig7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e5f7/5818869/6f3bc19e1a56/cmar-10-323Fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e5f7/5818869/2767db569db0/cmar-10-323Fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e5f7/5818869/22e0505f9c17/cmar-10-323Fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e5f7/5818869/b3e714612ac2/cmar-10-323Fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e5f7/5818869/e48ec28a3a66/cmar-10-323Fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e5f7/5818869/96c90653b1f6/cmar-10-323Fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e5f7/5818869/5b9ef91feb00/cmar-10-323Fig7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e5f7/5818869/6f3bc19e1a56/cmar-10-323Fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e5f7/5818869/2767db569db0/cmar-10-323Fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e5f7/5818869/22e0505f9c17/cmar-10-323Fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e5f7/5818869/b3e714612ac2/cmar-10-323Fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e5f7/5818869/e48ec28a3a66/cmar-10-323Fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e5f7/5818869/96c90653b1f6/cmar-10-323Fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e5f7/5818869/5b9ef91feb00/cmar-10-323Fig7.jpg

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