Dhahbi Joseph M, Atamna Hani, Selth Luke A
College of Medicine, California University of Science and Medicine, San Bernardino, CA, USA.
Dame Roma Mitchell Cancer Research Laboratories, Adelaide Medical School, The University of Adelaide, Adelaide, SA, Australia.
Biomark Cancer. 2018 Feb 20;10:1179299X18759545. doi: 10.1177/1179299X18759545. eCollection 2018.
Extracellular RNAs are gaining clinical interest as biofluid-based noninvasive markers for diseases, especially cancer. In particular, derivatives of transfer RNA (tRNA) are emerging as a new class of small-noncoding RNAs with high biomarker potential. We and others previously reported alterations in serum levels of specific tRNA halves in disease states including cancer. Here, we explored seminal fluid for tRNA halves as potential markers of prostate cancer. We found that 5' tRNA halves are abundant in seminal fluid and are elevated in prostate cancer relative to noncancer patients. Importantly, most of these tRNA halves are also detectable in prostatic tissues, and a subset were increased in malignant relative to adjacent normal tissue. These findings emphasize the potential of 5' tRNA halves as noninvasive markers for prostate cancer screening and diagnosis and provide leads for future work to elucidate a putative role of the 5' tRNA halves in carcinogenesis.
细胞外RNA作为基于生物流体的疾病（尤其是癌症）非侵入性标志物正引起临床关注。特别是，转运RNA（tRNA）的衍生物正在成为一类具有高生物标志物潜力的新型小非编码RNA。我们和其他人之前报道了在包括癌症在内的疾病状态下特定tRNA半体血清水平的改变。在这里，我们探索了精液中的tRNA半体作为前列腺癌的潜在标志物。我们发现5' tRNA半体在精液中含量丰富，并且相对于非癌症患者，在前列腺癌中有所升高。重要的是，这些tRNA半体中的大多数在前列腺组织中也可检测到，并且相对于相邻正常组织，一部分在恶性组织中增加。这些发现强调了5' tRNA半体作为前列腺癌筛查和诊断非侵入性标志物的潜力，并为未来阐明5' tRNA半体在致癌作用中假定作用的工作提供了线索。
