Fukutomi Y, Hashimoto T, Iwata M
Cell Immunol. 1987 Mar;105(1):187-98. doi: 10.1016/0008-8749(87)90067-0.
Normal mouse thymocytes activated with concanavalin A (Con A) released soluble factors which selectively inhibited rosette formation between human peripheral blood lymphocytes (PBL) and ox erythrocytes (Eo) sensitized with rabbit IgM antibodies. The factors were removed by absorption with mouse IgM-coupled Sepharose, and were recovered from the beads by elution at acid pH. They neither bound to mouse IgG-Sepharose nor inhibited rosette formation of PBL with Eo sensitized with rabbit IgG antibodies. Mouse IgM enhanced the formation of IgM-binding factors by Con A-activated thymocytes. Unstimulated normal mouse thymocytes also released IgM-binding factors upon incubation with mouse IgM. The molecular weights of IgM-binding factors were approximately 90-110 and 35-50 kDa as estimated by gel filtration. Each species of IgM-binding factors markedly suppressed the IgM-plaque-forming cell (PFC) response of sheep red blood cell-primed spleen cells and slightly suppressed the IgG PFC response.
用刀豆球蛋白A（Con A）激活的正常小鼠胸腺细胞释放出可溶性因子，这些因子能选择性抑制人外周血淋巴细胞（PBL）与经兔IgM抗体致敏的牛红细胞（Eo）之间的玫瑰花结形成。这些因子可通过与小鼠IgM偶联的琼脂糖亲和吸附去除，并在酸性pH条件下从珠子上洗脱回收。它们既不与小鼠IgG-琼脂糖结合，也不抑制PBL与经兔IgG抗体致敏的Eo的玫瑰花结形成。小鼠IgM增强了Con A激活的胸腺细胞产生IgM结合因子的能力。未受刺激的正常小鼠胸腺细胞在与小鼠IgM孵育后也会释放IgM结合因子。通过凝胶过滤估计，IgM结合因子的分子量约为90-110 kDa和35-50 kDa。每种IgM结合因子均显著抑制绵羊红细胞致敏的脾细胞的IgM空斑形成细胞（PFC）反应，并轻微抑制IgG PFC反应。
