Department of Chemistry, Faculty of Arts and Sciences, Kırıkkale University, Yahşihan, 71450 Kırıkkale, Turkey.
Department of Chemistry, Faculty of Arts and Sciences, Kırıkkale University, Yahşihan, 71450 Kırıkkale, Turkey.
Int J Biol Macromol. 2018 Jul 1;113:669-680. doi: 10.1016/j.ijbiomac.2018.02.155. Epub 2018 Feb 28.
The functionalization of polysaccharides with synthetic polymers has attracted great attention owing to its application in many industrial fields. The aim of this work was to study the impact of pectin functionalization with N,N-diethylacrylamide (DEAAm). Pectin was modified via microwave-induced graft copolymerization of DEAAm using ceric ammonium nitrate (CAN) and N,N,N',N'-tetramethylethylenediamine (TEMED). FTIR, C NMR, DSC/TGA, XRD, and SEM techniques were used to verify the structure of graft copolymers. Various reaction conditions such as microwave irradiation time, temperature, microwave power, monomer, initiator, and TEMED concentrations were investigated to get a maximum grafting yield of 192%. Lower critical solution temperatures (LCST) of graft copolymers were determined by UV spectroscopy. Graft copolymers were found to be thermo-sensitive, with LCST of 31°C and high thermal resistance. Biocompatibility test of copolymers showed that copolymers were not cytotoxic to L929 fibroblasts cells and can be used as a biomaterial.
由于多糖与合成聚合物的功能化在许多工业领域都有应用，因此引起了人们的极大关注。本工作旨在研究果胶与 N,N-二乙基丙烯酰胺（DEAAm）的功能化的影响。通过使用硝酸铈铵（CAN）和 N,N,N',N'-四甲基乙二胺（TEMED）的微波诱导接枝共聚反应对果胶进行修饰。使用傅里叶变换红外光谱（FTIR）、碳 NMR、差示扫描量热法/热重分析（DSC/TGA）、X 射线衍射（XRD）和扫描电子显微镜（SEM）技术来验证接枝共聚物的结构。研究了各种反应条件，如微波辐射时间、温度、微波功率、单体、引发剂和 TEMED 浓度，以获得最高接枝率 192%。通过紫外光谱法测定接枝共聚物的低临界溶液温度（LCST）。接枝共聚物具有温敏性，LCST 为 31°C，热稳定性高。共聚物的细胞相容性测试表明，共聚物对 L929 成纤维细胞无细胞毒性，可用作生物材料。
