Department of Ophthalmology, The Fourth Affiliated Hospital of China Medical University, Eye Hospital of China Medical University, Key Lens Research Laboratory of Liaoning Province, China; Department of Cell and Molecular Biology, Tulane University, New Orleans, LA 70118, USA.
Department of Cell and Molecular Biology, Tulane University, New Orleans, LA 70118, USA.
Exp Eye Res. 2018 May;170:169-176. doi: 10.1016/j.exer.2018.02.026. Epub 2018 Mar 1.
MicroRNA miR-126 has been shown to be required for proper angiogenesis in several models. However, its expression, regulation and function in the mouse choroid remain unclear. Our previous data has shown that miR-126 expression is enriched in the endothelial cells (ECs) of the mouse choroid. Here we report that a 5.5 kb Egfl7/miR-126 promoter drives the expression of miR-126 in the choroid ECs during choroidal vascular development. The expression of miR-126 in the ECs is regulated by flow stress likely through Krüppel-like transcriptional factors. miR-126 mice show mildly delayed choroidal vascular development, but adult knockout mice develop periphery choroidal vascular lesions. This study suggests that miR-126 is largely dispensable for mouse choroidal development but required for maintaining choroidal vasculature integrity.
miR-126 在几种模型中被证明是血管生成所必需的。然而，其在小鼠脉络膜中的表达、调控和功能仍不清楚。我们之前的数据表明，miR-126 的表达在小鼠脉络膜的内皮细胞（ECs）中富集。在这里，我们报告说，一个 5.5kb 的 Egfl7/miR-126 启动子在脉络膜血管发育过程中驱动 miR-126 在脉络膜 ECs 中的表达。ECs 中 miR-126 的表达受流体力的调节，可能通过 Krüppel 样转录因子。miR-126 小鼠表现出轻度延迟的脉络膜血管发育，但成年敲除小鼠则表现出周边脉络膜血管病变。这项研究表明，miR-126 对于小鼠脉络膜的发育不是必需的，但对于维持脉络膜血管的完整性是必需的。
