Department of Biomedical Engineering, Korea University, 466 Hana Science Hall, 145 Anamro, Seongbukgu, Seoul, 02841, Republic of Korea.
J Chromatogr A. 2018 Mar 30;1543:67-72. doi: 10.1016/j.chroma.2018.02.037. Epub 2018 Feb 21.
A microfluidic glass chip for performing sensitive mass spectrometry (MS) is developed by integrating nanochannel-based electropreconcentration with electrospray ionization (ESI). Cation analytes in acidic buffer conditions (∼pH 3) were preconcentrated at a micro-nano-microchannel junction where a positively charged polymer, polyE-323, is coated to reverse the electroosmotic flows (anodic EOF) and prevent sample adsorption. The preconcentrated cation analytes were delivered to a monolithic spray tip using an integrated field-free electrokinetic pump, enabling a stable positive ESI with negligible dead volume. The sample preconcentration-ESI-MS achieved one order of magnitude signal enhancement in MS signal with a peptide mixture.
一种用于进行灵敏质谱（MS）分析的微流控玻璃芯片，通过整合基于纳米通道的电预浓缩与电喷雾电离（ESI）技术而开发。在微-纳-微通道的交界处，带正电荷的聚合物聚 E-323 被涂覆以反转电动流（阳极EOF）并防止样品吸附，从而在酸性缓冲条件（约 pH 3）下对阳离子分析物进行电预浓缩。浓缩后的阳离子分析物使用集成的无场电动泵输送到整体式喷雾尖端，从而实现稳定的正 ESI，且死体积可忽略不计。与肽混合物相比，样品预浓缩-ESI-MS 可将 MS 信号的信号增强一个数量级。
