比较分枝杆菌基因组揭示了鸟分枝杆菌复合群的进化趋势。

Comparative genomics of Mycobacterium reveals evolutionary trends of M. avium complex.

机构信息

Bioinformatics Facility, Department of Botany, University of North Bengal, Darjeeling 734013, India.

Department of Microbiology, Vidyasagar University, Midnapore 721102, India.

出版信息

Genomics. 2019 May;111(3):426-435. doi: 10.1016/j.ygeno.2018.02.019. Epub 2018 Mar 1.

DOI:10.1016/j.ygeno.2018.02.019

PMID:29501678

Abstract

Mycobacterium is gram positive, slow growing, disease causing Actinobacteria. Beside potential pathogenic species, Mycobacterium also contains opportunistic pathogens as well as free living non-pathogenic species. Disease related various analyses on Mycobacterium tuberculosis are very widespread. However, genomic study of overall Mycobacterium species for understanding the selection pressure on genes as well as evolution of the organism is still illusive. MLSA and 16s rDNA based analysis has been generated for 241 Mycobacterium strains and a detailed analysis of codon and amino acid usage bias of mycobacterial genes, their functional analysis have been done. Further the evolutionary features of M. avium complex also have been revealed. Mycobacterial genes are moderately GC rich showed higher expression level in PPs and significant negative correlation with biosynthetic cost of proteins. Translational selection pressure was observed in mycobacterial genes. MAC showed close relationship with NPs and higher evolutionary rate in MAC revealed their constant evolving nature.

摘要

分枝杆菌是革兰氏阳性、生长缓慢、引起疾病的放线菌。除了潜在的致病物种外，分枝杆菌还包含机会性病原体以及自由生活的非致病性物种。分枝杆菌结核相关的各种分析非常广泛。然而，为了了解基因选择压力以及生物体进化，对整个分枝杆菌物种进行基因组研究仍然难以捉摸。已经对 241 株分枝杆菌菌株进行了 MLSA 和 16s rDNA 分析，并对分枝杆菌基因的密码子和氨基酸使用偏好性及其功能进行了详细分析。此外，还揭示了鸟分枝杆菌复合体的进化特征。分枝杆菌基因中度 GC 丰富，在 PPs 中表达水平较高，与蛋白质生物合成成本呈显著负相关。在分枝杆菌基因中观察到翻译选择压力。MAC 与 NPs 密切相关，MAC 中的进化率较高表明它们具有不断进化的性质。