Dendana F, Trabelsi H, Neiji S, Sellami H, Kammoun S, Makni F, Feki J, Cheikhrouhou F, Ayadi A
Laboratory of Fungal and Parasitic Molecular Biology, School of Medicine Sfax, Sfax University, Sfax, Tunisia.
Laboratory of Fungal and Parasitic Molecular Biology, School of Medicine Sfax, Sfax University, Sfax, Tunisia; Laboratory of Parasitology-Mycology, Habib Bourguiba University Sfax Hospital, Sfax, Tunisia.
Exp Parasitol. 2018 Apr;187:37-41. doi: 10.1016/j.exppara.2018.02.009. Epub 2018 Mar 1.
In the southern Tunisia Oasis, we conducted 211 water with drawals from various water traffic sites. This water is used for agriculture, swimming or various other human activities. Acanthamoeba genus was detected in 82% of collected samples. Sequencing of the amplification products with primers P892C/P892 has allowed us to detect genotypic variation with predominance of T4 genotype (51%) and presence of the genotypes T14, T5, T3, T16, T15, T10, T11, T9 and T7. They T4, T3, T5, T15, T11 and T10 genotypes have a high potential for pathogenicity and a very high degree of virulence due to their production of serine proteases and extracellular cysteine enzymes involved in tissue degradation of the host. T4 genotype was the most abundant in the environment as well as in infections caused by Acanthamoeba spp. T5 genotype was ranked second and T3 genotype was less abundant in the environment and its pathogenicity is discussed. Acanthamoeba strains with the genotypes T16, T9 and T7 were considered non pathogenic. In fact, they have been isolated only from the environment. However, for these strains, their role as a reservoir can be a real risk to human health.
在突尼斯南部绿洲，我们从各个取水点采集了211份水样。这些水用于农业、游泳或其他各种人类活动。在所采集的样本中，82%检测到棘阿米巴属。用引物P892C/P892对扩增产物进行测序，使我们能够检测到基因型变异，其中T4基因型占主导地位（51%），同时存在T14、T5、T3、T16、T15、T10、T11、T9和T7基因型。T4、T3、T5、T15、T11和T10基因型具有很高的致病潜力和极强的毒力，因为它们能产生参与宿主组织降解的丝氨酸蛋白酶和细胞外半胱氨酸酶。T4基因型在环境以及棘阿米巴属引起的感染中最为常见。T5基因型排名第二，T3基因型在环境中的含量较少，并对其致病性进行了讨论。基因型为T16、T9和T7的棘阿米巴菌株被认为无致病性。事实上，它们仅从环境中分离得到。然而，对于这些菌株，它们作为储存宿主的作用可能对人类健康构成真正的风险。
