State Key Laboratory of Silkworm Genome Biology, Key Laboratory for Sericulture Functional Genomics and Biotechnology of Agricultural Ministry, Southwest University, Chongqing 400715, China.
State Key Laboratory of Silkworm Genome Biology, Key Laboratory for Sericulture Functional Genomics and Biotechnology of Agricultural Ministry, Southwest University, Chongqing 400715, China.
Genomics. 2019 May;111(3):391-397. doi: 10.1016/j.ygeno.2018.02.014. Epub 2018 Mar 1.
Hippo signaling pathway (signaling pathway Hippo, hereinafter referred to as the Hippo pathway) was the earliest found in Drosophila (Schneck [1]), which can regulate the development of tissues and organs, even some components of the pathway were identified as tumor suppressor [2]. The pathway was more concerned in fruit flies and mice (Schneck [1]), but little attention has been given in silkworm, an important economic and lepidopteran model insect. In this manuscript, we identified major Hippo pathway related genes (Hippo, Salvador, Warts, Mats, Yorkie) in silkworm and named BmHpo, BmSav, BmWts, BmMats, BmYki. The domain organization of these genes was highly conserved in silkworm and other organisms suggesting that they could use similar protein-protein interactions to construct the Hippo kinase cascades. The expression profiles of these genes in silkworm during embryonic, larval, wandering, pupal and adult stages were analyzed, 14 tissues/organs of the day 3, 5th instar larvae (L5D3) as well. Experimental results showed that the expression of Hippo pathway had some influence on the development of silkworm. In order to find out the mechanism of Hippo pathway affecting silkworm development, BmHpo and BmYki were up-regulated and de-regulated in the cell line of Bombyx mori-BmN-SWU1(NS), and the changes of cell proliferation activity and cell cycle were detected. The distribution of BmYki was detected by immunofluorescence technique. This study provides insights into the genes of Hippo pathway which have a certain effect on somatic development and cell proliferation in silkworm.
Hippo 信号通路（信号通路 Hippo，以下简称 Hippo 通路）最早在果蝇中发现（Schneck [1]），可调节组织和器官的发育，甚至通路的某些成分被鉴定为肿瘤抑制因子 [2]。该通路在果蝇和小鼠中受到更多关注（Schneck [1]），但在重要的经济和鳞翅目模式昆虫家蚕中却很少受到关注。在本手稿中，我们在家蚕中鉴定了主要的 Hippo 通路相关基因（Hippo、Salvador、Warts、Mats、Yorkie），并将其命名为 BmHpo、BmSav、BmWts、BmMats、BmYki。这些基因在家蚕和其他生物中的结构域组织高度保守，表明它们可以利用类似的蛋白-蛋白相互作用来构建 Hippo 激酶级联。分析了这些基因在家蚕胚胎、幼虫、游走、蛹和成虫阶段以及 3 龄幼虫（L5D3）的 14 种组织/器官中的表达谱。实验结果表明 Hippo 通路的表达对家蚕的发育有一定的影响。为了找出 Hippo 通路影响家蚕发育的机制，我们在 Bombyx mori-BmN-SWU1(NS) 细胞系中上调和下调了 BmHpo 和 BmYki，并检测了细胞增殖活性和细胞周期的变化。通过免疫荧光技术检测了 BmYki 的分布。本研究为 Hippo 通路基因在家蚕体发育和细胞增殖方面具有一定作用提供了新的见解。
