Fujii T, Igarashi T, Kishimoto S
J Natl Cancer Inst. 1987 Mar;78(3):509-17.
The in vivo significance of suppressor macrophages for antitumor immunity was investigated in a syngeneic tumor system. The presence of suppressor macrophages in the spleens of X5563 C3H/HeN tumor-bearing mice (TBM) was directly shown in vitro. Thus the addition of splenic macrophages of TBM suppressed the in vitro secondary induction of both tumor-specific cytotoxic T-cells and effector cells of the delayed-type hypersensitivity reaction. Splenic macrophages of TBM exerted suppression on tumor-specific T-cell-mediated cytotoxicity in the effector phase as well. Prostaglandin production was found to be one of the major mechanisms involved in macrophage-induced suppression. In vivo treatment of TBM with carrageenan and/or indomethacin retarded tumor growth and in parallel augmented cell-mediated cytotoxicity against X5563 cells, probably by affecting suppressor macrophages in vivo. The suppressive effect of splenic macrophages from TBM was clearly demonstrated in a tumor-neutralization test indicating that suppressor macrophages were able to exert their function in vivo as well as in vitro. All these results suggested that suppressor macrophages had in vivo significance for the suppression of the immunosurveillance of the hosts.
在同基因肿瘤系统中研究了抑制性巨噬细胞在抗肿瘤免疫中的体内意义。在体外直接证实了X5563 C3H/HeN荷瘤小鼠（TBM）脾脏中存在抑制性巨噬细胞。因此，添加TBM的脾巨噬细胞可抑制肿瘤特异性细胞毒性T细胞和迟发型超敏反应效应细胞的体外二次诱导。TBM的脾巨噬细胞在效应阶段也对肿瘤特异性T细胞介导的细胞毒性产生抑制作用。发现前列腺素的产生是巨噬细胞诱导的抑制作用的主要机制之一。用角叉菜胶和/或吲哚美辛对TBM进行体内治疗可延缓肿瘤生长，同时增强针对X5563细胞的细胞介导的细胞毒性，这可能是通过影响体内的抑制性巨噬细胞实现的。在肿瘤中和试验中清楚地证明了TBM脾巨噬细胞的抑制作用，表明抑制性巨噬细胞能够在体内和体外发挥其功能。所有这些结果表明，抑制性巨噬细胞在体内对宿主免疫监视的抑制具有重要意义。
